Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-EL-M3063
文献（1052）
说明书

说明书

别称：

DIF、Tnfa、TNF-a、TNFSF2、Tnlg1f、Tnfsf1a、TNFalpha、TNF-alpha、Tnf、Cachectin、Tumor necrosis factor、Tumor necrosis factor ligand superfamily member 2

Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Mouse
检测范围： 7.81-500 pg/mL
灵敏度： 4.69 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Mouse
检测方法	比色法;ELISA;夹心法
检测范围	7.81-500 pg/mL
灵敏度	4.69 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Lercanidipine Enhances Cisplatin Activity: Dual Anticancer and Anti-Inflammatory Effects via Caspase Activation and MAPK Inhibition

IF: 4.3

Journal： Pharmaceuticals (2025)

DOI：10.3390/ph18050651

Reactivity：Mouse
Deciphering the Antidepressant Potential of Piper betle L. Essential Oil and Its Inclusion Complex: Network Pharmacology and Experimental Insights

IF: 4.3

Journal： MOLECULAR NEUROBIOLOGY (2025)

DOI：10.1007/s12035-025-05117-8

Reactivity：Mouse

Sample Type：serum
Phochinenin I, a Bioactive Constituent from Pholidota chinensis, Attenuates Inflammatory Response and Oxidative Stress In Vitro and In Vivo

IF: 4.3

Journal： ACS Omega (2025)

DOI：10.1021/acsomega.4c11614

Reactivity：Mouse
Disulfiram alleviates immune-mediated liver injury by inhibiting pyroptosis in hepatocytes through the NF-κB pathway

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2025)

DOI：10.1016/j.ejphar.2025.177352

Reactivity：Mouse

Sample Type：serum
Diacerein's Antiproliferative Effects Alone and with 5-Fluorouracil in an Ehrlich Solid Tumour Model: Molecular Docking, Molecular Dynamics Simulation Studies, and Experimental Verification

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2025)

DOI：10.1016/j.ejphar.2025.177564

Reactivity：Mouse

Sample Type：Ehrlich Ascites carcinoma (EAC) cell-Xenograft
Protective Effects of Qingre Sanjie Jiaonang on Pulmonary Fibrosis: A Pilot Study

IF: 4.2

Journal： Journal of Inflammation Research (2025)

DOI：10.2147/JIR.S479432

Reactivity：Mouse

Sample Type：bronchoalveolar lavage fluid (BALF),serum
Mechanism of berbamine-mediated DNA damage in synovial fibroblasts to alleviate rheumatoid arthritis

IF: 4.2

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2025)

DOI：10.1016/j.ejphar.2025.177597

Reactivity：Mouse
Exploring the role of the CD74+ cardiac macrophage subset in trastuzumab cardiotoxicity and its mechanisms

IF: 4.2

Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2025)

DOI：10.1016/j.bbadis.2025.167875

Reactivity：Mouse

Sample Type：serum
Colon-Targeted Mucoadhesive PLGA Microspheres Loaded with Ramulus Mori Alkaloids for Enhanced Water-Soluble Drug Delivery in Ulcerative Colitis Treatment

IF: 4.2

Journal： MOLECULES (2025)

DOI：10.3390/molecules30091878

Reactivity：Mouse

Sample Type：serum
HNF-1α promotes urethral fibrosis by up-regulating STAT3 transcriptional activity in mice hypospadias

IF: 4.2

Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2025)

DOI：10.1016/j.bbadis.2025.167893

Reactivity：Mouse

Sample Type：penile tissue

Species

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Canine

Human

Mouse

Porcine

Rat

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