Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Circular RNA expression profile of liver tissues in an EtOH-induced mouse model of alcoholic hepatitis
IF: 3.170Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2019)PMID：31493407Reactivity：MouseSample Type：Serum,Tissue homogenate -
Pancreastatin inhibitor PSTi8 attenuates hyperinsulinemia induced obesity and inflammation mediated insulin resistance via MAPK/NOX3-JNK pathway
IF: 3.170 -
MMI-0100 Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice through Targeting MK2 Pathway
IF: 3.060 -
Design, Synthesis and Investigation of the Potential Anti-Inflammatory Activity of 7-O-Amide Hesperetin Derivatives
IF: 3.060 -
Gastrodin attenuates neuroinflammation in DOI-induce Tourette syndrome in rats
IF: 2.965Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019)PMID：30790395Reactivity：RatSample Type：Serum,Tissue homogenate -
Gastrodin alleviates Tourette syndrome via Nrf-2/HO-1/HMGB1/NF-кB pathway
IF: 2.965Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019)PMID：31468582Reactivity：RatSample Type：Tissue homogenate -
Sinomenine Attenuates Cartilage Degeneration by Regulating miR-223-3p/NLRP3 Inflammasome Signaling
IF: 2.939Journal： INFLAMMATION (2019)PMID：30847744Reactivity：MouseSample Type：Serum,Cell culture supernatant -
Effect of Oral T2 Antigen on Chronic Prostatitis/Chronic Pelvic Pain Syndrome in Mice Model
IF: 2.939 -
Role of Interleukin-17 in Pathogenesis of Intestinal Fibrosis in Mice
IF: 2.937 -
Formononetin Ameliorates Cognitive Disorder via PGC-1α Pathway in Neuroinflammation Conditions in High-Fat Diet-Induced Mice
IF: 2.761Journal： CNS & Neurological Disorders-Drug Targets (2019)PMID：31389320Reactivity：MouseSample Type：hippocampus
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
