Mouse ACTH(Adrenocorticotropic Hormone) ELISA Kit
中文名称：小鼠促肾上腺皮质激素(ACTH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠ACTH抗原包被于酶标板上，实验时样品或标准品中的小鼠ACTH与包被的小鼠ACTH竞争生物素标记的抗小鼠ACTH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠ACTH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠ACTH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠ACTH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Human PERIOD3 variants lead to winter depression-like behaviours via glucocorticoid signalling
IF: 18.900Journal： Nature Metabolism (2024)Reactivity：MouseSample Type：Plasma -
Probiotics administration alleviates cognitive impairment and circadian rhythm disturbance induced by sleep deprivation
IF: 5.600Journal： Food Science and Human Wellness (2024)Reactivity：MouseSample Type：Serum -
The Activation of p300 Enhances the Sensitivity of Pituitary Adenomas to Dopamine Agonist Treatment by Regulating the Transcription of DRD2
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：Mouse -
New polycyclic polyprenylated acylphloroglucinols with antidepressant activities from Hypericum perforatum L.
IF: 4.500Journal： BIOORGANIC CHEMISTRY (2024)Reactivity：MouseSample Type：serum -
Bacillus coagulans and Clostridium butyricum synergistically alleviate depression in a chronic unpredictable mild stress mouse model through altering gut microbiota and prefrontal cortex gene expression
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：serum,prefrontal cortex (PFC) -
Dysregulation of the 3β-hydroxysteroid dehydrogenase type 2 enzyme and steroid hormone biosynthesis in chronic kidney disease
IF: 3.900Journal： Frontiers in Endocrinology (2024)Reactivity：MouseSample Type：plasma -
Role and mechanism of PVN–sympathetic–adipose circuit in depression and insulin resistance induced by chronic stress
IF: 7.700 -
Betaine prevents cognitive dysfunction by suppressing hippocampal microglial activation in chronic social isolated male mice
IF: 7.200Journal： PHYTOTHERAPY RESEARCH (2023)DOI：10.1002/ptr.7944PMID：37846157Reactivity：MouseSample Type：plasma -
Metformin inhibits cell proliferation and ACTH secretion in AtT20?cells via regulating the MAPK pathway
IF: 4.100 -
Effects of constant darkness on behaviour and physiology of male and female mice
IF: 3.400
实验操作视频
