Mouse ALB(Albumin) ELISA Kit
中文名称：小鼠白蛋白(ALB)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 62.5-4000 ng/mL
- 灵敏度： 37.5 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠ALB抗体包被于酶标板上，实验时样品（或标准品）中的小鼠ALB会与包被抗体结合。后依次加入生物素化的抗小鼠ALB抗体和辣根过氧化物酶标记的亲和素，抗小鼠ALB抗体与结合在包被抗体上的小鼠ALB结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ALB浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ALB的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 ng/mL |
| 灵敏度 | 37.5 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠ALB，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A single-domain antibody targeting factor XII inhibits both thrombosis and inflammation
IF: 14.700Journal： Nature Communications (2024)Reactivity：MouseSample Type：urine tissue -
Lactate drives epithelial-mesenchymal transition in diabetic kidney disease via the H3K14la/KLF5 pathway
IF: 10.700 -
LncRNA PVT1 induces mitochondrial dysfunction of podocytes via TRIM56 in diabetic kidney disease
IF: 8.100Journal： Cell Death & Disease (2024)Reactivity：MouseSample Type：urine tissue -
Engineering an energy-dissipating hybrid tissue in?vivo for obesity treatment
IF: 7.500 -
A novel antidiuretic hormone governs tumour-induced renal dysfunction
IF: 64.800 -
α1-adrenoceptor stimulation ameliorates lipopolysaccharide-induced lung injury by inhibiting alveolar macrophage inflammatory responses through NF-κB and ERK1/2 pathway in ARDS
IF: 7.300Journal： Frontiers in Immunology (2023)PMID：36685596Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
GPR43 deficiency protects against podocyte insulin resistance in diabetic nephropathy through the restoration of AMPKα activity
IF: 11.556 -
Oestradiol promotes the intrahepatic bile duct development of C57BL/6CrSlc mice during embryonic period via Notch signalling pathway
IF: 5.310 -
Supplement of microbiota-accessible carbohydrates prevents neuroinflammation and cognitive decline by improving the gut microbiota-brain axis in diet-induced obese mice
IF: 5.793Journal： Journal of Neuroinflammation (2020)PMID：32127019Reactivity：MouseSample Type：Fecal pellet -
α2A-adrenoceptor deficiency attenuates lipopolysaccharide-induced lung injury by increasing norepinephrine levels and inhibiting alveolar macrophage activation in acute respiratory distress syndrome
IF: 5.223
实验操作视频
