Mouse AMH(Anti-Mullerian Hormone) ELISA Kit
中文名称:小鼠抗缪勒管激素(AMH)酶联免疫吸附测定试剂盒
别称:

Price:
Size:
96 T48 T96 T × 5
- 反应性: Mouse
- 检测范围: 0.78-50 ng/mL
- 灵敏度: 0.47 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠AMH抗体包被于酶标板上,实验时样品(或标准品)中的小鼠AMH会与包被抗体结合。后依次加入生物素化的抗小鼠AMH抗体和辣根过氧化物酶标记的亲和素,抗小鼠AMH抗体与结合在包被抗体上的小鼠AMH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,AMH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中AMH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 | 96 T/ 48 T/ 96 T × 5 |
反应时间 | 3 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.78-50 ng/mL |
灵敏度 | 0.47 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠AMH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
Proanthocyanidins protects 3-NPA-induced ovarian function decline by activating SESTRIN2-NRF2-mediated oxidative stress in mice
IF: 3.800Human umbilical cord mesenchymal stem cell-derived extracellular vesicles harboring IGF-1 improve ovarian function of mice with premature ovarian insufficiency through the Nrf2/HO-1 pathway
IF: 3.800Journal: Journal of Ovarian Research (2024)Reactivity:MouseSample Type:serumMaternal androgen exposure induces intergenerational effects via paternal inheritance
IF: 3.400The ovarian-related effects of polystyrene nanoplastics on human ovarian granulosa cells and female mice
IF: 6.800Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)PMID:37087970Reactivity:MouseSample Type:serumAtorvastatin exerts dual effects of lesion regression and ovarian protection in the prevention and treatment of endometriosis
IF: 5.000The emerging role of lysine succinylation in ovarian aging
IF: 4.400Journal: Reproductive Biology and Endocrinology (2023)PMID:37081483Reactivity:MouseSample Type:serumIron deposition in ovarian endometriosis evaluated by magnetic resonance imaging R2* correlates with ovarian function
IF: 4.000Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice
IF: 3.600Journal: AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2023)PMID:36752682Reactivity:MouseSample Type:bloodMelatonin prevents cyclophosphamide-induced primordial follicle loss by inhibiting ovarian granulosa cell apoptosis and maintaining AMH expression
IF: 6.055Distinctions in PCOS Induced by Letrozole Vs Dehydroepiandrosterone With High-fat Diet in Mouse Model
IF: 5.051