Mouse AMH(Anti-Mullerian Hormone) ELISA Kit
中文名称：小鼠抗缪勒管激素(AMH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠AMH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠AMH会与包被抗体结合。后依次加入生物素化的抗小鼠AMH抗体和辣根过氧化物酶标记的亲和素，抗小鼠AMH抗体与结合在包被抗体上的小鼠AMH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，AMH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中AMH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠AMH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Ubiquitin-like modifier 1 ligating enzyme 1 relieves cisplatin-induced premature ovarian failure by reducing endoplasmic reticulum stress in granulosa cells
IF: 4.982Journal： Reproductive Biology and Endocrinology (2022)PMID：35610622Reactivity：MouseSample Type：ovarian tissue -
The effect of icariin on autoimmune premature ovarian insufficiency via modulation of Nrf2/HO-1/Sirt1 pathway in mice
IF: 2.089 -
Anti-tuberculosis drugs used in a directly observed treatment short course (DOTS) schedule alter endocrine patterns and reduce the ovarian reserve and oocyte quality in the mouse
IF: 1.973Journal： REPRODUCTION FERTILITY AND DEVELOPMENT (2022)DOI：10.1071/RD22108PMID：36219878Reactivity：MouseSample Type：serum -
The effect of Bu Shen Huo Xue Tang on autoimmune premature ovarian insufficiency via Modulation of the Nrf2/Keap1 signaling pathway in mice
IF: 4.360 -
Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice
IF: 4.285 -
Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide.
IF: 2.916 -
Bisphenol A promotes autophagy in ovarian granulosa cells by inducing AMPK/mTOR/ULK1 signalling pathway
IF: 7.577Journal： ENVIRONMENT INTERNATIONAL (2020)PMID：33387880Reactivity：MouseSample Type：Cell culture supernatant, Serum -
Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles
IF: 3.998 -
Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency
IF: 2.823Journal： MOLECULAR REPRODUCTION AND DEVELOPMENT (2020)PMID：32741069Reactivity：MouseSample Type：Serum -
Vinorelbine Potently Induces Placental Cell Death, Does Not Harm Fertility and is a Potential Treatment for Ectopic Pregnancy
IF: 6.183
实验操作视频
