Mouse Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit
中文名称:小鼠血管紧张素Ⅱ(Ang-Ⅱ)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用小鼠Ang-Ⅱ抗原包被于酶标板上,实验时样品或标准品中的小鼠Ang-Ⅱ与包被的小鼠Ang-Ⅱ竞争生物素标记的抗小鼠Ang-Ⅱ单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,小鼠Ang-Ⅱ浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中小鼠Ang-Ⅱ的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠Ang-Ⅱ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Circadian light/dark cycle reversal exacerbates the progression of chronic kidney disease in mice
IF: 8.300 -
ACE2 deficiency inhibits thoracic aortic dissection by enhancing SIRT3 mediated inhibition of inflammation and VSCMs phenotypic switch
IF: 6.000Journal: MOLECULAR MEDICINE (2024)Reactivity:MouseSample Type:peripheral blood plasma -
Synergistic effect of collagen peptide LR-7 and taurine on the prevention of cardiovascular injury in high salt-induced hypertensive mice
IF: 4.800Journal: Food Bioscience (2024)Reactivity:MouseSample Type:heart tissue -
Silenced long non-coding RNA RMST ameliorates cardiac dysfunction and inflammatory response in doxorubicin-induced heart failure in C57BL/6 mice via the modulation of the microRNA-10b-5p/TRAF6 axis
IF: 3.700Journal: JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY (2024)Reactivity:MouseSample Type:serum -
Salidroside Pre-Treatment Inhibits Hypertensive Renal Injury and Fibrosis Through Inhibiting Wnt/β-Catenin Pathway
IF: 2.300Journal: Dose-Response (2024)Reactivity:MouseSample Type:Serum,kidney tissue -
Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1–7)-Mas-mediated macrophage polarization
IF: 11.400 -
Effect of Lactobacillus casei fermented milk on fracture healing in osteoporotic mice
IF: 6.055Journal: Frontiers in Endocrinology (2022)Reactivity:MouseSample Type:blood -
Bone morphogenetic protein 4 in perivascular adipose tissue ameliorates hypertension through regulation of angiotensinogen
IF: 5.846Journal: Frontiers in Cardiovascular Medicine (2022)PMID:36457800Reactivity:MouseSample Type:plasma -
LncRNA HOX transcript antisense RNA mitigates cardiac function injury in chronic heart failure via regulating microRNA-30a-5p to target KDM3A
IF: 5.295Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022)PMID:35083842Reactivity:MouseSample Type:plasma -
Short-Term Hyperuricemia Leads to Structural Retinal Changes That Can be Reversed by Serum Uric Acid Lowering Agents in Mice
IF: 4.925Journal: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022)PMID:36098977Reactivity:MouseSample Type:serum