Mouse ANP(Atrial Natriuretic Peptide) ELISA Kit
中文名称：小鼠心钠肽(ANP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠ANP抗原包被于酶标板上，实验时样品或标准品中的小鼠ANP与包被的小鼠ANP竞争生物素标记的抗小鼠ANP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠ANP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠ANP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠ANP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Impact of prolonged exposure to occasional and regular waterpipe smoke on cardiac injury, oxidative stress and mitochondrial dysfunction in male mice
IF: 4.000 -
Neferine mitigates angiotensin II-induced atrial fibrillation and fibrosis via upregulation of Nrf2/HO-1 and inhibition of TGF-β/p-Smad2/3 pathways
IF: 3.900 -
Corilagin Alleviates Ang II-Induced Cardiac Fibrosis by Regulating the PTEN/AKT/mTOR Pathway
IF: 2.300 -
Paeoniflorin Inhibits Atrial Fibrosis and Atrial Fibrillation in Angiotensin II-Infused Mice Through the PI3K-Akt Pathway
IF: 2.300 -
The muscle-enriched myokine Musclin impairs beige fat thermogenesis and systemic energy homeostasis via Tfr1/PKA signaling in male mice
IF: 16.600 -
Porcupine inhibitor CGX1321 alleviates heart failure with preserved ejection fraction in mice by blocking WNT signaling
IF: 7.169Journal： ACTA PHARMACOLOGICA SINICA (2022)PMID：36473990PMC10203103Reactivity：MouseSample Type：serum -
Knockout of cardiac troponin I-interacting kinase leads to cardiac dysfunction and remodelling
IF: 2.963Journal： CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2022)PMID：35781726Reactivity：MouseSample Type：serum -
Recombinant Soluble Corin Improves Cardiac Function in Mouse Models of Heart Failure
IF: 5.501Journal： Journal of the American Heart Association (2021)PMID：33759549Reactivity：MouseSample Type：Plasma -
Arterial smooth muscle cell PKD2 (TRPP1) channels regulate systemic blood pressure
IF: 7.616 -
Nonstructural Protein 1 (NS1)-Mediated Inhibition of c-Abl Results in Acute Lung Injury and Priming for Bacterial Co-infections: Insights Into 1918 H1N1 Pandemic?
IF: 5.778
实验操作视频
