Mouse ApoB(Apolipoprotein B) ELISA Kit
中文名称：小鼠载脂蛋白B(ApoB)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠ApoB抗体包被于酶标板上，实验时样品（或标准品）中的小鼠ApoB会与包被抗体结合。后依次加入生物素化的抗小鼠ApoB抗体和辣根过氧化物酶标记的亲和素，抗小鼠ApoB抗体与结合在包被抗体上的小鼠ApoB结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ApoB浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ApoB的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠ApoB，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A gut-derived hormone regulates cholesterol metabolism
IF: 64.500 -
Alpha Hydroxyl Acids from Mume Fructus and Schisandrae Chinensis Fructus Prevent Obesity by Inhibiting Intestinal Lipase in Diet-Induced Obese Mice
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：Serum -
Hepatic MDM2 Causes Metabolic Associated Fatty Liver Disease by Blocking Triglyceride-VLDL Secretion via ApoB Degradation
IF: 17.521 -
Visual Identification of Trichosporon asahii, a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe
IF: 8.008Journal： ANALYTICAL CHEMISTRY (2022)PMID：35920602Reactivity：MouseSample Type：hepatic tissue,epididymal fat tissue -
Hepatic cholesterol synthesis and lipoprotein levels impaired by dietary fructose and saturated fatty acids in mice: Insight on PCSK9 and CD36
IF: 3.639 -
Liraglutide Reduces Postprandial Hyperlipidemia by Increasing ApoB48 (Apolipoprotein B48) Catabolism and by Reducing ApoB48 Production in Patients With Type 2 Diabetes Mellitus
IF: 6.086Journal： ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY (2018)PMID：30026275Reactivity：MouseSample Type：Serum -
High-Resolution Genetic Mapping in the Diversity Outbred Mouse Population Identifies Apobec1 as a Candidate Gene for Atherosclerosis
IF: 2.511
实验操作视频
