Mouse ApoE(Apolipoprotein E) ELISA Kit
中文名称：小鼠载脂蛋白E(ApoE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠ApoE抗体包被于酶标板上，实验时样品（或标准品）中的小鼠ApoE会与包被抗体结合。后依次加入生物素化的抗小鼠ApoE抗体和辣根过氧化物酶标记的亲和素，抗小鼠ApoE抗体与结合在包被抗体上的小鼠ApoE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ApoE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ApoE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠ApoE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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KaiXinSan improves learning and memory impairment by regulating cholesterol homeostasis in mice overloaded with 27-OHC
IF: 2.700Journal： JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2024)Reactivity：MouseSample Type：brain tissue -
Melatonin Suppresses Macrophage M1 Polarization and ROS-Mediated Pyroptosis via Activating ApoE/LDLR Pathway in Influenza A-Induced Acute Lung Injury.
IF: 7.310Journal： Oxidative Medicine and Cellular Longevity (2022)PMID：36425057Reactivity：MouseSample Type：serum -
Effects of toxic apolipoprotein E fragments on Tau phosphorylation and cognitive impairment in neonatal mice under sevoflurane anesthesia
IF: 3.405 -
Coenzyme Q10 alleviates sevoflurane?induced neuroinflammation by regulating the levels of apolipoprotein E and phosphorylated tau protein in mouse hippocampal neurons
IF: 2.100Journal： Molecular Medicine Reports (2020)PMID：32377738Reactivity：MouseSample Type：Tissue homogenate -
Protective effects of Coenzyme Q10 against sevoflurane-induced cognitive impairment through regulating apolipoprotein E and phosphorylated Tau expression in young mice
IF: 1.911Journal： INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE (2020)PMID：32473608Reactivity：MouseSample Type：Hippocampus
实验操作视频
