Mouse BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
中文名称：小鼠脑源性神经营养因子(BDNF)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠BDNF抗体包被于酶标板上，实验时样品（或标准品）中的小鼠BDNF会与包被抗体结合。后依次加入生物素化的抗小鼠BDNF抗体和辣根过氧化物酶标记的亲和素，抗小鼠BDNF抗体与结合在包被抗体上的小鼠BDNF结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，BDNF浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中BDNF的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠BDNF，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Osteocalcin-expressing neutrophils from skull bone marrow exert immunosuppressive and neuroprotective effects after TBI
IF: 7.500 -
BDNF augmentation reverses cranial radiation therapy-induced cognitive decline and neurodegenerative consequences
IF: 6.200Journal： Acta Neuropathologica Communications (2024)Reactivity：MouseSample Type：Brain hippocampal tissue -
Treatment of Parkinson's disease model with human umbilical cord mesenchymal stem cell-derived exosomes loaded with BDNF
IF: 5.200Journal： LIFE SCIENCES (2024)Reactivity：MouseSample Type：brain tissue -
NR1H4 ameliorates Parkinson’s disease via inhibiting astrocyte activation and neuroinflammation in a CEBPβ/NF-κB dependent manner
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：midbrain tissue -
Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：brain tissue -
Effective action of silymarin against ketamine-induced schizophrenia in male mice: Insight into the biochemical and molecular mechanisms of action
IF: 3.700Journal： JOURNAL OF PSYCHIATRIC RESEARCH (2024)Reactivity：MouseSample Type：striatum tissue,prefrontal cortex tissue,hippocampus tissue -
Concomitant Exposure to Lower Doses of Arsenic, Lead, and Manganese Induces Greater Synergistic Neurotoxicity Than Individual Metals in Mice
IF: 3.400Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2024)Reactivity：MouseSample Type：Brain Tissue -
Lutein improves remyelination by reducing of neuroinflammation in C57BL/6 mouse models of multiple sclerosis
IF: 3.400 -
Ginkgolide B as a biopsychosocial treatment salvages repeated restraint stress-induced amygdalar anomalies in mice
IF: 2.000Journal： IBRO Neuroscience Reports (2024)Reactivity：MouseSample Type：Serum -
Self-immolative nanocapsules precisely regulate depressive neuronal microenvironment for synergistic antidepression therapy
IF: 10.200
实验操作视频
