Mouse BNP(Brain Natriuretic Peptide) ELISA Kit
中文名称：小鼠脑钠素(BNP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠BNP抗原包被于酶标板上，实验时样品或标准品中的小鼠BNP与包被的小鼠BNP竞争生物素标记的抗小鼠BNP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠BNP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠BNP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠BNP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms
IF: 14.903 -
Porcupine inhibitor CGX1321 alleviates heart failure with preserved ejection fraction in mice by blocking WNT signaling
IF: 7.169Journal： ACTA PHARMACOLOGICA SINICA (2022)PMID：36473990PMC10203103Reactivity：MouseSample Type：serum -
Role of the Gut Microbiota in Glucose Metabolism During Heart Failure
IF: 5.846Journal： Frontiers in Cardiovascular Medicine (2022)PMID：35859583Reactivity：MouseSample Type：Serum -
LncRNA HOX transcript antisense RNA mitigates cardiac function injury in chronic heart failure via regulating microRNA-30a-5p to target KDM3A
IF: 5.295Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022)PMID：35083842Reactivity：MouseSample Type：plasma -
Long-Term Sleep Deprivation-Induced Myocardial Remodeling and Mitochondrial Dysfunction in Mice Were Attenuated by Lipoic Acid and N-Acetylcysteine
IF: 5.215 -
Tetrahydrobiopterin (BH4) Supplementation Prevents the Cardiorenal Effects of Diabetes in Mice by Reducing Oxidative Stress, Inflammation and Fibrosis
IF: 4.757 -
Knockout of cardiac troponin I-interacting kinase leads to cardiac dysfunction and remodelling
IF: 2.963Journal： CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2022)PMID：35781726Reactivity：MouseSample Type：serum -
Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity
IF: 6.530Journal： BIOMEDICINE & PHARMACOTHERAPY (2021)PMID：34311530Reactivity：Human,MouseSample Type：Serum -
MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis
IF: 6.530 -
microRNA-193-3p attenuates myocardial injury of mice with sepsis via STAT3/HMGB1 axis
IF: 5.531
实验操作视频
