Mouse CGRP1(Calcitonin Gene Related Peptide 1) ELISA Kit
中文名称：小鼠降钙素基因相关肽1(CGRP1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠CGRP1抗体包被于酶标板上，实验时样品（或标准品）中的小鼠CGRP1会与包被抗体结合。后依次加入生物素化的抗小鼠CGRP1抗体和辣根过氧化物酶标记的亲和素，抗小鼠CGRP1抗体与结合在包被抗体上的小鼠CGRP1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CGRP1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CGRP1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠CGRP1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Nociceptive adenosine A2A receptor on trigeminal nerves orchestrates CGRP release to regulate the progression of oral squamous cell carcinoma
IF: 10.800Journal： International Journal of Oral Science (2024)Reactivity：MouseSample Type：serum -
ILC2-derived CGRP triggers acute inflammation and nociceptive responses in bacterial cystitis
IF: 7.500Journal： Cell Reports (2024)Reactivity：MouseSample Type：bladder tissue -
An-Gong-Niu-Huang-Wan (AGNHW) regulates cerebral blood flow by improving hypoperfusion, cerebrovascular reactivity and microcirculation disturbances after stroke
IF: 5.300Journal： Chinese Medicine (2024)Reactivity：MouseSample Type：serum,brain homogenates -
AMPK/MTOR/TP53 Signaling Pathway Regulation by Calcitonin Gene-Related Peptide Reduces Oxygen-Induced Lung Damage in Neonatal Rats through Autophagy Promotion
IF: 5.100 -
Cancer cells co-opt nociceptive nerves to thrive in nutrient-poor environments and upon nutrient-starvation therapies
IF: 31.373 -
C-Fiber Degeneration Enhances Alveolar Macrophage-Mediated IFN-α/β Response to Respiratory Syncytial Virus
IF: 9.043Journal： Microbiology Spectrum (2022)Reactivity：MouseSample Type：colon tissue -
Nociceptor Neurons are Involved in the Host Response to Escherichia coli Urinary Tract Infections
IF: 4.631Journal： Journal of Inflammation Research (2022)PMID：35702548Reactivity：MouseSample Type：Bladder tissue -
Chemogenetic modulation of sensory neurons reveals their regulating role in melanoma progression
IF: 7.801Journal： Acta Neuropathologica Communications (2021)Reactivity：MouseSample Type：B16-F10 cell-Xenograft -
Study on the Chemical Constituents and Anti-Migraine Activity of Supercritical CO2 Extracts of Zanthoxylum schinifolium
IF: 5.811Journal： Frontiers in Pharmacology (2021)Reactivity：MouseSample Type：serum -
Dibutyl phthalate aggravated asthma-like symptoms through oxidative stress and increasing calcitonin gene-related peptide release
IF: 4.872Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020)PMID：32446102Reactivity：MouseSample Type：Tissue homogenate
实验操作视频
