Mouse CRH(Corticotropin Releasing Hormone) ELISA Kit
中文名称：小鼠促肾上腺皮质激素释放激素(CRH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠CRH抗原包被于酶标板上，实验时样品或标准品中的小鼠CRH与包被的小鼠CRH竞争生物素标记的抗小鼠CRH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠CRH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠CRH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠CRH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Hippocampal Crhr1 conditional gene knockout ameliorated the depression-like behavior and pathological damage in male offspring mice caused by chronic stress during pregnancy
IF: 2.600Journal： BEHAVIOURAL BRAIN RESEARCH (2024)Reactivity：MouseSample Type：serum -
Uncarialins J—M from Uncaria rhynchophylla and Their Anti-depression Mechanism in Unpredictable Chronic Mild Stress-Induced Mice via Activating 5-HT1A Receptor
IF: 6.000Journal： CHINESE JOURNAL OF CHEMISTRY (2021)Reactivity：MouseSample Type：lung -
The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation.
IF: 4.380Journal： Scientific Reports (2021)PMID：33542312Reactivity：MouseSample Type：Serum,Tissue homogenate(hypothalamus,hypophysis ) -
Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics
IF: 4.268 -
High activity of the endogenous opioid system and acute but not chronic stress influence experimental colitis development in mice.
IF: 2.478Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018)PMID：30683828Reactivity：MouseSample Type：plasma -
Microbiota Modulate Anxiety-Like Behavior and Endocrine Abnormalities in Hypothalamic-Pituitary-Adrenal Axis
IF: 4.300Journal： Frontiers in Cellular and Infection Microbiology (2017)PMID：29250490Reactivity：MouseSample Type：Tissue homogenate
实验操作视频
