Mouse Cys-C(Cystatin C) ELISA Kit
中文名称：小鼠胱抑素C(Cys-C)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠Cys-C抗体包被于酶标板上，实验时样品（或标准品）中的小鼠Cys-C会与包被抗体结合。后依次加入生物素化的抗小鼠Cys-C抗体和辣根过氧化物酶标记的亲和素，抗小鼠Cys-C抗体与结合在包被抗体上的小鼠Cys-C结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Cys-C浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Cys-C的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠Cys-C，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Dynamic changes in the real-time glomerular filtration rate and kidney injury markers in different acute kidney injury models
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：kidney tissue -
Unveiling the role of mtDNA in Liver-Kidney Crosstalk: Insights from trichloroethylene hypersensitivity syndrome
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum -
The STING antagonist SN-011 ameliorates cisplatin induced acute kidney injury via suppression of STING/NF-κB-mediated inflammation
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Plasma -
Berberine alleviated contrast-induced acute kidney injury by mitophagy-mediated NLRP3 inflammasome inactivation in a mice model
IF: 3.300Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Investigation of nephrotoxicity on mice exposed to polystyrene nanoplastics and the potential amelioration effects of DHA-enriched phosphatidylserine
IF: 9.800 -
Targeting the TWEAK–Fn14 pathway prevents dysfunction in cardiac calcium handling after acute kidney injury
IF: 7.300 -
Catalpol ameliorates inflammation and oxidative stress via regulating Sirt1 and activating Nrf2/HO-1 signaling against acute kidney injury
IF: 4.500 -
Investigation of the potential ameliorative effects of DHA-enriched phosphatidylserine on bisphenol A-induced murine nephrotoxicity
IF: 4.300Journal： FOOD AND CHEMICAL TOXICOLOGY (2023)PMID：37666289Reactivity：MouseSample Type：kidney tissues -
The role of microbial metabolites in diabetic kidney disease
IF: 4.000
实验操作视频
