Mouse D2D(D-Dimer) ELISA Kit
中文名称：小鼠D-二聚体(D2D)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 78.13-5000 ng/mL
- 灵敏度： 46.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠D2D抗原包被于酶标板上，实验时样品或标准品中的小鼠D2D与包被的小鼠D2D竞争生物素标记的抗小鼠D2D单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠D2D浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠D2D的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 78.13-5000 ng/mL |
| 灵敏度 | 46.88 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血浆 |
| 特异性 | 可检测样本中的小鼠D2D，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A single-domain antibody targeting factor XII inhibits both thrombosis and inflammation
IF: 14.700Journal： Nature Communications (2024)Reactivity：MouseSample Type：plasma -
Red Blood Cell Membrane-Coated Nanoparticles Enable Incompatible Blood Transfusions
IF: 14.300 -
YOD1 protects against MRSA sepsis-induced DIC through Lys33-linked deubiquitination of NLRP3
IF: 8.100Journal： Cell Death & Disease (2024)Reactivity：MouseSample Type：plasma -
Tissue-type plasminogen activator (tPA) homozygous Tyr471His mutation associates with thromboembolic disease
IF: 9.900 -
Administration of spermidine attenuates concanavalin A-induced liver injury
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：36724559Reactivity：MouseSample Type：serum -
Programmable DARPin-based receptors for the detection of thrombotic markers
IF: 16.174 -
CD44–fibrinogen binding promotes bleeding in acute promyelocytic leukemia by in situ fibrin(ogen) deposition
IF: 7.637 -
The effect of shock duration on trauma-induced coagulopathy in a murine model
Journal： Intensive Care Medicine Experimental (2022)PMID：34993669Reactivity：MouseSample Type：blood -
Consumptive coagulopathy of severe yellow fever occurs independently of hepatocellular tropism and massive hepatic injury
IF: 9.412Journal： PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)PMID：33268494Reactivity：MouseSample Type：plasma -
Scavenger receptor A1 attenuates aortic dissection via promoting efferocytosis in macrophages
IF: 4.825
实验操作视频
