Mouse D-LDH(D-Lactate Dehydrogenase) ELISA Kit
中文名称：小鼠D-乳酸脱氢酶(D-LDH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 4.69-300 mIU/mL
- 灵敏度： 2.81 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠D-LDH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠D-LDH会与包被抗体结合。后依次加入生物素化的抗小鼠D-LDH抗体和辣根过氧化物酶标记的亲和素，抗小鼠D-LDH抗体与结合在包被抗体上的小鼠D-LDH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，D-LDH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中D-LDH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 4.69-300 mIU/mL |
| 灵敏度 | 2.81 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠D-LDH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Targeted H2S-Mediated Gas Therapy with pH-Sensitive Release Property for Myocardial Ischemia–Reperfusion Injury by Platelet Membrane
IF: 8.100 -
S1PR2 participates in intestinal injury in severe acute pancreatitis by regulating macrophage pyroptosis
IF: 5.700Journal： Frontiers in Immunology (2024)Reactivity：MouseSample Type：serum -
An Amino Acids and Dipeptide Injection Inhibits the TNF-α/HMGB1 Inflammatory Signaling Pathway to Reduce Pyroptosis and M1 Microglial Polarization in POCD Mice: the Gut to the Brain
IF: 4.600Journal： MOLECULAR NEUROBIOLOGY (2024)Reactivity：MouseSample Type：serum -
EPHB2 Knockdown Mitigated Myocardial Infarction by Inhibiting MAPK Signaling
IF: 3.200 -
TFAM-Mediated mitochondrial transfer of MSCs improved the permeability barrier in sepsis-associated acute lung injury
IF: 7.200 -
Lactobacillus paracasei influences the gut-microbiota-targeted metabolic modulation of the immune status of diarrheal mice
IF: 6.100 -
In-vitro and in-vivo assessment of pH-responsive core–shell nanocarrier system for sequential delivery of methotrexate and 5-fluorouracil for the treatment of breast cancer
IF: 5.800Journal： INTERNATIONAL JOURNAL OF PHARMACEUTICS (2023)PMID：37972670Reactivity：MouseSample Type：Serum -
Rutin prevents pyroptosis and M1 microglia via Nrf2/Mac-1/caspase-1-mediated inflammasome axis to improve POCD
IF: 5.600 -
Hmgcs2 regulates M2 polarization of macrophages to repair myocardial injury induced by sepsis
IF: 5.200 -
Stress granules inhibit endoplasmic reticulum stress-mediated apoptosis during hypoxia-induced injury in acute liver failure
IF: 4.300
实验操作视频
