Mouse DAO(Diamine Oxidase) ELISA Kit
中文名称：小鼠二胺氧化酶(DAO)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.91-250 ng/mL
- 灵敏度： 2.35 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠DAO抗体包被于酶标板上，实验时样品（或标准品）中的小鼠DAO会与包被抗体结合。后依次加入生物素化的抗小鼠DAO抗体和辣根过氧化物酶标记的亲和素，抗小鼠DAO抗体与结合在包被抗体上的小鼠DAO结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，DAO浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中DAO的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.91-250 ng/mL |
| 灵敏度 | 2.35 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠DAO，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A preventative role of nitrate for hypoxia-induced intestinal injury
IF: 7.400 -
Exopolysaccharides and Surface-Layer Proteins Expressed by Biofilm-State Lactiplantibacillus plantarum Y42 Play Crucial Role in Preventing Intestinal Barrier and Immunity Dysfunction of Balb/C Mice Infected by Listeria monocytogenes ATCC 19115
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：serum -
Partially hydrolyzed guar gum attenuates broad-spectrum antibiotic-induced gut microbiota disruption associated with intestinal inflammation and barrier damage in mice
IF: 4.800 -
Lactobacillus paracasei influences the gut-microbiota-targeted metabolic modulation of the immune status of diarrheal mice
IF: 6.100 -
Atractyloside-A ameliorates spleen deficiency diarrhea by interfering with TLR4/MyD88/NF-κB signaling activation and regulating intestinal flora homeostasis
IF: 5.714 -
Lactobacillus paracasei from koumiss ameliorates diarrhea in mice via tight junctions modulation
IF: 4.893 -
Electroacupuncture modulates gut microbiota in mice: A potential target in postoperative cognitive dysfunction
IF: 2.227Journal： Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology (2022)DOI：10.1002/ar.25065PMID：36094150Reactivity：MouseSample Type：Hippocampal tissue -
Cryptotanshinone alleviates chemotherapy-induced colitis in mice with colon cancer via regulating fecal-bacteria-related lipid metabolism
IF: 5.893 -
Dihydrotanshinone attenuates chemotherapy-induced intestinal mucositis and alters fecal microbiota in mice
IF: 4.545 -
Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model
IF: 4.225
实验操作视频
