Mouse EGF(Epidermal Growth Factor) ELISA Kit
中文名称：小鼠表皮生长因子(EGF)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠EGF抗体包被于酶标板上，实验时样品（或标准品）中的小鼠EGF会与包被抗体结合。后依次加入生物素化的抗小鼠EGF抗体和辣根过氧化物酶标记的亲和素，抗小鼠EGF抗体与结合在包被抗体上的小鼠EGF结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，EGF浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中EGF的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠EGF，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Accelerated intestinal wound healing via dual electrostimulation from a soft and biodegradable electronic bandage
IF: 34.300Journal： Nature Electronics (2024)Reactivity：MouseSample Type：intestinal tissue -
Engineering an energy-dissipating hybrid tissue in?vivo for obesity treatment
IF: 7.500 -
Exopolysaccharides and Surface-Layer Proteins Expressed by Biofilm-State Lactiplantibacillus plantarum Y42 Play Crucial Role in Preventing Intestinal Barrier and Immunity Dysfunction of Balb/C Mice Infected by Listeria monocytogenes ATCC 19115
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：serum -
Lactobacillus paracasei influences the gut-microbiota-targeted metabolic modulation of the immune status of diarrheal mice
IF: 6.100 -
Inhibition of Tumor Growth and Modulation of Antioxidant Activity of Rhodoxanthin Isolated from Taxus baccata Aril against B16F10 Murine Malignant Melanoma
IF: 7.675 -
Wound-healing activity of glycoproteins from white jade snail (Achatina fulica) on experimentally burned mice
IF: 6.953Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)PMID：33539960Reactivity：MouseSample Type：Serum -
Effects of catalpol on asthma by airway remodeling via inhibiting TGF-β1 and EGF in ovalbumin-induced asthmatic mice
IF: 3.375Journal： American Journal of Translational Research (2020)DOI：PMID:32774762PMID：32774762Reactivity：MouseSample Type：serum, BALF -
Autograft microskin combined with adipose-derived stem cell enhances wound healing in a full-thickness skin defect mouse model
IF: 4.627 -
ADAM12 induces EMT and promotes cell migration, invasion and proliferation in pituitary adenomas via EGFR/ERK signaling pathway
IF: 2.759Journal： BIOMEDICINE & PHARMACOTHERAPY (2017)PMID：29136943Reactivity：MouseSample Type：Cell culture supernatant -
Impact of Helicobacter pylori on the healing process of the gastric barrier
IF: 2.787Journal： WORLD JOURNAL OF GASTROENTEROLOGY (2016)PMID：27672275Reactivity：MouseSample Type：Cell culture supernatant
实验操作视频
