Mouse ET-1(Endothelin 1) ELISA Kit
中文名称：小鼠内皮素1(ET-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.78-50 pg/mL
- 灵敏度： 0.47 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠ET-1抗体包被于酶标板上，实验时样品（或标准品）中的小鼠ET-1会与包被抗体结合。后依次加入生物素化的抗小鼠ET-1抗体和辣根过氧化物酶标记的亲和素，抗小鼠ET-1抗体与结合在包被抗体上的小鼠ET-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ET-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ET-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 pg/mL |
| 灵敏度 | 0.47 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠ET-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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TNF-α-downregulated SIRT1 regulates mitochondrial-autophagy and apoptosis in renal vascular endothelial cells involved in trichloroethylene-induced immune kidney injury
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：urine tissue -
Role of neutrophil myeloperoxidase in the development and progression of high-altitude pulmonary edema
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)PMID：38382360Reactivity：MouseSample Type：lung tissue -
The Chinese medicine Fufang Zhenzhu Tiaozhi capsule protects against atherosclerosis by suppressing EndMT via modulating Akt1/β-catenin signaling pathway
IF: 5.195 -
Biejiajian Pill Ameliorates Diabetes-Associated Atherosclerosis through Inhibition of the NLRP3 Inflammasome
IF: 2.650Journal： Evidence-based Complementary and Alternative Medicine (2022)PMID：35692570Reactivity：MouseSample Type：serum -
Endothelin-1 down-regulated vascular endothelial growth factor A is involved in trichloroethene-induced kidney injury
IF: 1.851Journal： TOXICOLOGY AND INDUSTRIAL HEALTH (2022)Reactivity：MouseSample Type：serum -
Ginsenoside Rg1-Notoginsenoside R1-Protocatechuic Aldehyde Reduces Atherosclerosis and Attenuates Low-Shear Stress-Induced Vascular Endothelial Cell Dysfunction
IF: 5.811 -
Study on the Chemical Constituents and Anti-Migraine Activity of Supercritical CO2 Extracts of Zanthoxylum schinifolium
IF: 5.811Journal： Frontiers in Pharmacology (2021)Reactivity：MouseSample Type：serum -
Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors
IF: 6.126 -
miR-1929-3p Overexpression Alleviates Murine Cytomegalovirus-Induced Hypertensive Myocardial Remodeling by Suppressing Ednra/NLRP3 Inflammasome Activation
IF: 2.276Journal： Biomed Research International (2020)PMID：33457411Reactivity：MouseSample Type：serum, heart -
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy
IF: 8.063Journal： Theranostics (2019)PMID：31281522Reactivity：MouseSample Type：mammary gland tumor tissue,plasma
实验操作视频
