Mouse FE(Ferritin) ELISA Kit
中文名称：小鼠铁蛋白(FE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠FE抗体包被于酶标板上，实验时样品（或标准品）中的小鼠FE会与包被抗体结合。后依次加入生物素化的抗小鼠FE抗体和辣根过氧化物酶标记的亲和素，抗小鼠FE抗体与结合在包被抗体上的小鼠FE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，FE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中FE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠FE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Integrative clinical and preclinical studies identify FerroTerminator1 as a potent therapeutic drug for MASH
IF: 27.700 -
Effect of ferric citrate on hippocampal iron accumulation and widespread molecular alterations associated with cognitive disorder in an ovariectomized mice model
IF: 4.800Journal： CNS Neuroscience & Therapeutics (2024)Reactivity：MouseSample Type：serum -
The effectiveness of sanggenon c in alleviating SLC7A11-induced ferroptosis in lung cancer was evaluated using in vivo, in vitro, and computational approaches
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：blood -
Intermittent hypoxia exacerbates metabolic dysfunction-associated fatty liver disease by aggravating hepatic copper deficiency-induced ferroptosis
IF: 4.400 -
Single-Cell RNA-seq Analysis Reveals a Positive Correlation between Ferroptosis and Beta-Cell Dedifferentiation in Type 2 Diabetes
IF: 3.900 -
Lif-deficiency promote systemic Iron metabolism disorders and increases the susceptibility of osteoblasts to ferroptosis
IF: 3.500 -
MYOCD and SRF-mediated MLCK transcription prevents polymorphonuclear neutrophils from ferroptosis in sepsis-related acute lung injury
IF: 3.300 -
Social defeat stress impairs systemic iron metabolism by activating the hepcidin–ferroportin axis
IF: 2.500 -
High-Altitude Hypoxia Induces Excessive Erythrocytosis in Mice via Upregulation of the Intestinal HIF2a/Iron-Metabolism Pathway
IF: 4.700 -
Exploring the role of ferroptosis in the doxorubicin-induced chronic cardiotoxicity using a murine model
IF: 5.168Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2022)PMID：35667395Reactivity：MouseSample Type：cardiac , liver tissue
实验操作视频
