Mouse FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称：小鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠FSH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠FSH会与包被抗体结合。后依次加入生物素化的抗小鼠FSH抗体和辣根过氧化物酶标记的亲和素，抗小鼠FSH抗体与结合在包被抗体上的小鼠FSH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，FSH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中FSH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠FSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Metformin protects ovarian granulosa cells in chemotherapy-induced premature ovarian failure mice through AMPK/PPAR-γ/SIRT1 pathway
IF: 4.600 -
Linking alterations in estrogen receptor expression to memory deficits and depressive behavior in an ovariectomy mouse model
IF: 4.600 -
Liraglutide improved the reproductive function of obese mice by upregulating the testicular AC3/cAMP/PKA pathway
IF: 4.400Journal： Reproductive Biology and Endocrinology (2024)PMID：38509558Reactivity：MouseSample Type：serum -
GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion
IF: 4.400 -
Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome
IF: 4.200Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024)Reactivity：MouseSample Type：ovarian tissue -
Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy
IF: 4.000 -
Progestin and adipoQ receptor 7 (PAQR7) mediate the anti-apoptotic effect of P4 on human granulosa cells and its deficiency reduces ovarian function in female mice
IF: 4.000 -
Proanthocyanidins protects 3-NPA-induced ovarian function decline by activating SESTRIN2-NRF2-mediated oxidative stress in mice
IF: 3.800Journal： Scientific Reports (2024)Reactivity：MouseSample Type：blood -
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles harboring IGF-1 improve ovarian function of mice with premature ovarian insufficiency through the Nrf2/HO-1 pathway
IF: 3.800Journal： Journal of Ovarian Research (2024)Reactivity：MouseSample Type：serum -
Genetic loss of Uchl1 leads to female infertility by affecting oocyte quality and follicular development
IF: 3.800Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2024)Reactivity：MouseSample Type：serum
实验操作视频
