Mouse FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称:小鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠FSH抗体包被于酶标板上,实验时样品(或标准品)中的小鼠FSH会与包被抗体结合。后依次加入生物素化的抗小鼠FSH抗体和辣根过氧化物酶标记的亲和素,抗小鼠FSH抗体与结合在包被抗体上的小鼠FSH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,FSH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中FSH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠FSH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Apelin receptor modulation mitigates letrozole-induced polycystic ovarian pathogenesis in mice
IF: 3.700 -
Effects of chronic CuNPs treatment followed by termination for two spermatogenic cycles in the testicular functions of mice
IF: 3.300Journal: REPRODUCTIVE TOXICOLOGY (2024)Reactivity:MouseSample Type:serum -
Mixed exposure to PFOA and PFOS induces oocyte apoptosis and subfertility in mice by activating the Hippo signaling pathway
IF: 3.300Journal: REPRODUCTIVE TOXICOLOGY (2024)Reactivity:MouseSample Type:Serum -
Sub-acute bisphenol A exposure induces proteomic alterations and impairs male reproductive health in mice
IF: 3.200Journal: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2024)Reactivity:MouseSample Type:serum -
Deoxynivalenol induces ovarian damage and uterine changes in prepubertal and adult mice
IF: 2.600 -
Hyperandrogenemia elevates expression of apelin and apelin receptor protein in the mice pituitary
IF: 1.900Journal: Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2024)DOI:10.1002/jez.2857Reactivity:MouseSample Type:serum -
Shenqi Qiangjing Granules Ameliorate Asthenozoospermia in Mice by Regulating Ferroptosis through the METTL3/GPX4 Signaling Axis
IF: 1.700Journal: TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE (2024)Reactivity:MouseSample Type:serum -
Regulation of Ovary Function in Aged Mice by Reactive Oygen Species through the PI3K/Akt/mTOR Pathway
IF: 0.500 -
Dichloroacetic acid and trichloroacetic acid as disinfection by-products in drinking water are endocrine-disrupting chemicals
IF: 13.600 -
Red blood cell extracellular vesicles deliver therapeutic siRNAs to skeletal muscles for treatment of cancer cachexia
IF: 12.400