Mouse FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称:小鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠FSH抗体包被于酶标板上,实验时样品(或标准品)中的小鼠FSH会与包被抗体结合。后依次加入生物素化的抗小鼠FSH抗体和辣根过氧化物酶标记的亲和素,抗小鼠FSH抗体与结合在包被抗体上的小鼠FSH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,FSH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中FSH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠FSH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Deletion of Fbxw7 in oocytes causes follicle loss and premature ovarian insufficiency in mice
IF: 4.3Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2024)Reactivity:MouseSample Type:plasma -
Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome
IF: 4.2Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024)Reactivity:MouseSample Type:ovarian tissue -
Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy
IF: 4.0 -
Progestin and adipoQ receptor 7 (PAQR7) mediate the anti-apoptotic effect of P4 on human granulosa cells and its deficiency reduces ovarian function in female mice
IF: 4.0 -
Proanthocyanidins protects 3-NPA-induced ovarian function decline by activating SESTRIN2-NRF2-mediated oxidative stress in mice
IF: 3.8Journal: Scientific Reports (2024)Reactivity:MouseSample Type:blood -
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles harboring IGF-1 improve ovarian function of mice with premature ovarian insufficiency through the Nrf2/HO-1 pathway
IF: 3.8Journal: Journal of Ovarian Research (2024)Reactivity:MouseSample Type:serum -
Genetic loss of Uchl1 leads to female infertility by affecting oocyte quality and follicular development
IF: 3.8Journal: MOLECULAR AND CELLULAR ENDOCRINOLOGY (2024)Reactivity:MouseSample Type:serum -
Apelin receptor modulation mitigates letrozole-induced polycystic ovarian pathogenesis in mice
IF: 3.7 -
Effects of chronic CuNPs treatment followed by termination for two spermatogenic cycles in the testicular functions of mice
IF: 3.3Journal: REPRODUCTIVE TOXICOLOGY (2024)Reactivity:MouseSample Type:serum -
Mixed exposure to PFOA and PFOS induces oocyte apoptosis and subfertility in mice by activating the Hippo signaling pathway
IF: 3.3Journal: REPRODUCTIVE TOXICOLOGY (2024)Reactivity:MouseSample Type:Serum

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