Mouse FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称：小鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠FSH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠FSH会与包被抗体结合。后依次加入生物素化的抗小鼠FSH抗体和辣根过氧化物酶标记的亲和素，抗小鼠FSH抗体与结合在包被抗体上的小鼠FSH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，FSH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中FSH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠FSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Therapeutic Effects of Melatonin in Female Mice with Central Precocious Puberty by Regulating the Hypothalamic Kiss-1/Kiss1R System
IF: 2.700 -
Exosomes from adipose-derived stem cells alleviate premature ovarian failure via blockage of autophagy and AMPK/mTOR pathway
IF: 2.700 -
Indirubin-3’-oxime promotes the efficacy of GnRHa in obese-induced central precocious puberty and maintains normal bone growth and body weight via the ERK-Sp1-KISS-1/GPR54 axis
IF: 1.600 -
Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway
IF: 9.112 -
Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice
IF: 8.101Journal： FREE RADICAL BIOLOGY AND MEDICINE (2022)PMID：36309297Reactivity：MouseSample Type：testicular tissues -
Tilapia skin peptides restore cyclophosphamide-induced premature ovarian failure via inhibiting oxidative stress and apoptosis in mice
IF: 6.317 -
Role of EGFR expressed on the granulosa cells in the pathogenesis of polycystic ovarian syndrome
IF: 6.055 -
Treatment of copper nanoparticles (CuNPs) for two spermatogenic cycles impairs testicular activity via down-regulating steroid receptors and inhibition of germ cell proliferation in a mice model
IF: 5.881 -
Distinctions in PCOS Induced by Letrozole Vs Dehydroepiandrosterone With High-fat Diet in Mouse Model
IF: 5.051 -
Ubiquitin-like modifier 1 ligating enzyme 1 relieves cisplatin-induced premature ovarian failure by reducing endoplasmic reticulum stress in granulosa cells
IF: 4.982Journal： Reproductive Biology and Endocrinology (2022)PMID：35610622Reactivity：MouseSample Type：ovarian tissue
实验操作视频
