Mouse GHRL(Ghrelin) ELISA Kit
中文名称：小鼠饥饿素(GHRL)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Mouse
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠GHRL抗原包被于酶标板上，实验时样品或标准品中的小鼠GHRL与包被的小鼠GHRL竞争生物素标记的抗小鼠GHRL单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠GHRL浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠GHRL的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 2 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠GHRL，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
The use of capsicum oleoresin microparticles to mitigate hepatic damage and metabolic disorders induced by obesity
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IF: 5.900Activation of ectopic olfactory receptor 544 induces GLP-1 secretion and regulates gut inflammation
IF: 10.245Heracleum dissectum Ledeb. ethanol extract attenuates metabolic syndrome symptoms in high-fat diet-induced obese mice by activating adiponectin/AMPK signaling
IF: 4.451Journal： Journal of Functional Foods (2021)Reactivity：MouseSample Type：bloodAsprosin, a novel glucogenic adipokine: a potential therapeutic implication in diabetes mellitus
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IF: 5.309Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels
IF: 3.647
