Mouse GROα/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
中文名称：小鼠生长调节致癌基因α(GROα/CXCL1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠GROα/CXCL1抗体包被于酶标板上，实验时样品（或标准品）中的小鼠GROα/CXCL1会与包被抗体结合。后依次加入生物素化的抗小鼠GROα/CXCL1抗体和辣根过氧化物酶标记的亲和素，抗小鼠GROα/CXCL1抗体与结合在包被抗体上的小鼠GROα/CXCL1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，GROα/CXCL1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中GROα/CXCL1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠GROα/CXCL1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury
IF: 14.300Journal： Advanced Science (2024)Reactivity：MouseSample Type：kidney tissue -
LECs regulate neutrophil clearance through IL-17RC/CMTM4/NF-κB axis at sites of inflammation or infection
IF: 7.900Journal： Mucosal Immunology (2024)Reactivity：MouseSample Type：ear skin tissue -
Mesenchymal stromal cells alleviate APAP-induced liver injury via extracellular vesicle-mediated regulation of the miR-186-5p/CXCL1 axis
IF: 7.100Journal： Stem Cell Research & Therapy (2024)Reactivity：MouseSample Type：liver tissue -
Sea buckthorn extract mitigates chronic obstructive pulmonary disease by suppression of ferroptosis via scavenging ROS and blocking p53/MAPK pathways
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Mitoxantrone attenuates lipopolysaccharide-induced acute lung injury via inhibition of NEDD8 activating enzyme
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
The novel pleuromutilin derivative 22–((4-((4-nitrophenyl)acetamido)phenyl)thio)deoxy pleuromutilin possesses robust anti-mycoplasma activity both in vitro and in vivo
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis
IF: 4.966Journal： FASEB JOURNAL (2020)PMID：32862457Reactivity：MouseSample Type：Cell culture supernatant,Tissue homogenate,serum -
Wu-tou decoction attenuates neuropathic pain via suppressing spinal astrocytic IL-1R1/TRAF6/JNK signaling
IF: 5.168 -
Molecular mechanisms of the analgesic action of Wu-tou Decoction on neuropathic pain in mice revealed using microarray and network analysis
IF: 3.223Journal： ACTA PHARMACOLOGICA SINICA (2017)PMID：28816231Reactivity：MouseSample Type：Tissue homogenate -
P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy
IF: 12.413
实验操作视频
