Mouse Hepc(Hepcidin) ELISA Kit
中文名称：小鼠铁调素(Hepc)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Mouse
- 检测范围： 125-8000 pg/mL
- 灵敏度： 75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠Hepc抗体包被于酶标板上，实验时样品（或标准品）中的小鼠Hepc会与包被抗体结合。后依次加入生物素化的抗小鼠Hepc抗体和辣根过氧化物酶标记的亲和素，抗小鼠Hepc抗体与结合在包被抗体上的小鼠Hepc结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Hepc浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Hepc的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 125-8000 pg/mL |
| 灵敏度 | 75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠Hepc，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Interferon-induced polarization of M1 macrophages mediates antiviral activity against the hepatitis B virus via the hepcidin-ferroportin axis
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serumEffect of ferric citrate on hippocampal iron accumulation and widespread molecular alterations associated with cognitive disorder in an ovariectomized mice model
IF: 4.800Journal： CNS Neuroscience & Therapeutics (2024)Reactivity：MouseSample Type：serumIron accumulation induced by hepcidin1 knockout accelerates the progression of aging osteoporosis
IF: 2.600Journal： Journal of Orthopaedic Surgery and Research (2024)PMID：38216929Reactivity：MouseSample Type：SerumHypoxia-inducible factor upregulation by roxadustat attenuates drug reward by altering brain iron homoeostasis
IF: 39.300Journal： Signal Transduction and Targeted Therapy (2023)PMID：37718358Reactivity：MouseSample Type：liver,serumIntracellular Iron Accumulation Induces Inflammatory and Oxidative Status of the Host After Japanese Encephalitis Viral Infection
IF: 5.100Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：35408955Reactivity：MouseSample Type：serumA Reproducible Mouse Model of Moderate CKD With Early Manifestations of Osteoblastic Transition of Cardiovascular System
IF: 4.755Epigallocatechin-3-Gallate Suppresses BMP-6-Mediated SMAD1/5/8 Transactivation of Hepcidin Gene by Inducing SMILE in Hepatocytes
IF: 6.313Dysregulation of iron homeostasis and methamphetamine reward behaviors in Clk1-deficient mice
IF: 6.150Journal： ACTA PHARMACOLOGICA SINICA (2021)Reactivity：MouseSample Type：brain tissuePerinatal and early-life cobalt exposure impairs essential metal metabolism in immature ICR mice
IF: 6.025
