Mouse I-PTH(intact Parathormone) ELISA Kit
中文名称：小鼠全段甲状旁腺素(I-PTH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠I-PTH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠I-PTH会与包被抗体结合。后依次加入生物素化的抗小鼠I-PTH抗体和辣根过氧化物酶标记的亲和素，抗小鼠I-PTH抗体与结合在包被抗体上的小鼠I-PTH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，I-PTH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中I-PTH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠I-PTH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Role of sphingolipid metabolism signaling in a novel mouse model of renal osteodystrophy based on transcriptomic approach
IF: 7.500Journal： CHINESE MEDICAL JOURNAL (2024)Reactivity：MouseSample Type：Serum -
Vascular, valvular and kidney calcification manifested in mouse models of adenine-induced chronic kidney disease
IF: 3.000 -
Fucoidan Attenuated Kidney and Bone Damage Caused by CKD-MBD in Mice by Upregulating Klotho
IF: 0.700Journal： Pharmacognosy Magazine (2023)Reactivity：MouseSample Type：serum -
A tissue-selective estrogen complex as treatment of osteoporosis in experimental lupus.
IF: 2.858 -
Role of Gut Microbiota, Gut-Brain and Gut Liver Axes in Physiological Regulation of Inflammation, Energy Balance, and Metabolism: Pasteurized Akkermansia muciniphila protects from fat mass gain but not from bone loss
IF: 3.469Journal： AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2020)PMID：31961709Reactivity：MouseSample Type：Serum -
High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：29545872Reactivity：MouseSample Type：Serum -
Adenovirus-expressing miR-153-3p alleviates aortic calcification in a rat model with chronic kidney disease
IF: 1.706Journal： International Journal of Clinical and Experimental Pathology (2017)DOI：PMID:31966509PMID：31966509Reactivity：RatSample Type：serum
实验操作视频
