Mouse IFNα(Interferon Alpha) ELISA Kit
中文名称：小鼠干扰素α(IFNα)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFNα抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFNα会与包被抗体结合。后依次加入生物素化的抗小鼠IFNα抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFNα抗体与结合在包被抗体上的小鼠IFNα结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFNα浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFNα的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IFNα，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively
IF: 9.000Journal： EMBO Molecular Medicine (2024)Reactivity：MouseSample Type：plasma -
The neuroprotective effects of caffeic acid phenethyl ester against methamphetamine-induced neurotoxicity
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024) -
Interferon-induced polarization of M1 macrophages mediates antiviral activity against the hepatitis B virus via the hepcidin-ferroportin axis
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
A novel oral TLR7 agonist orchestrates immune response and synergizes with PD-L1 blockade via type I IFN pathway in lung cancer
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：LLC cell-Xenograft -
Helicobacter pylori activates SLFN4+MDSCs to accelerate gastric intestinal metaplasia
IF: 4.600 -
The Impact of Chemical Modifications on the Interferon-Inducing and Antiproliferative Activity of Short Double-Stranded Immunostimulating RNA
IF: 4.200 -
Enhancing the bioavailability of quercetin via the construction of carboxymethylated curdlan/quercetin nanocomplex
IF: 10.700 -
Type 1 interferon mediated signaling is indispensable for eliciting anti-tumor responses by Mycobacterium indicus pranii
IF: 7.300Journal： Frontiers in Immunology (2023)PMID：37122749Reactivity：MouseSample Type：Tumor draining lymph nodes，B16F10 cell-xenograft -
STING deficiency protects against wasp venom-induced acute kidney injury
IF: 6.700 -
FBXO6 regulates the antiviral immune responses via mediating alveolar macrophages survival
IF: 20.693
实验操作视频
