Mouse IFN-β(Interferon Beta) ELISA Kit
中文名称：小鼠β干扰素(IFN-β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFN-β会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFN-β抗体与结合在包被抗体上的小鼠IFN-β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IFN-β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Interplay Between Sialic Acids, Siglec-E, and Neu1 Regulates MyD88- and TRIF-Dependent Pathways for TLR4-Activation During Leishmania donovani Infection
IF: 7.561Journal： Frontiers in Immunology (2021)PMID：33763070Reactivity：MouseSample Type：Cell culture supernatant -
Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3
IF: 7.397Journal： IMMUNOLOGY (2021)PMID：33513265Reactivity：Human,MouseSample Type：Cell（HEK293T cells,the mouseperitoneal macrophage cells） -
Chitosan-Poly(Acrylic Acid) Nanoparticles Loaded with R848 and MnCl2 Inhibit Melanoma via Regulating Macrophage Polarization and Dendritic Cell Maturation
IF: 6.400Journal： International Journal of Nanomedicine (2021)PMID：34456564Reactivity：MouseSample Type：serum -
Tanreqing Inhibits LPS-Induced Acute Lung Injury In Vivo and In Vitro Through Downregulating STING Signaling Pathway
IF: 5.811Journal： Frontiers in Pharmacology (2021)Reactivity：MouseSample Type：serum -
OTUD1 Negatively Regulates Type I IFN Induction by Disrupting Noncanonical Ubiquitination of IRF3
IF: 4.886 -
DDIT3 targets innate immunity via the DDIT3-OTUD1-MAVS pathway to promote BVDV replication
IF: 4.501 -
ADP-ribosyltransferase PARP11 modulates the interferon antiviral response by mono-ADP-ribosylating the ubiquitin E3 ligase β-TrCP
IF: 14.633 -
Terminating the renewal of tumor-associated macrophages: A sialic acid-based targeted delivery strategy for cancer immunotherapy
IF: 4.213Journal： INTERNATIONAL JOURNAL OF PHARMACEUTICS (2019)PMID：31593811Reactivity：MouseSample Type：Tissue homogenate -
Induction of OTUD1 by RNA viruses potently inhibits innate immune responses by promoting degradation of the MAVS/TRAF3/TRAF6 signalosome
IF: 6.158 -
miR-194 Inhibits Innate Antiviral Immunity by Targeting FGF2 in Influenza H1N1 Virus Infection
IF: 4.076Journal： Frontiers in Microbiology (2017)PMID：29163456Reactivity：Human,MouseSample Type：Cell culture supernatant,Bronchoalveolar Lavage Fluid
实验操作视频
