Mouse IFN-γ(Interferon Gamma) ELISA Kit
中文名称：小鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFN-γ抗体与结合在包被抗体上的小鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Staphylococcal enterotoxin M causes intestine dysfunction via activating inflammation
IF: 1.275 -
Role of receptor-interacting protein 1/receptor-interacting protein 3 in inflammation and necrosis following chronic constriction injury of the sciatic nerve
IF: 1.266 -
Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis
IF: 5.168 -
Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice
IF: 4.259Journal： Scientific Reports (2017)PMID：28225024Reactivity：MouseSample Type：Bronchoalveolar Lavage Fluid,Serum -
Oral Immunization with Recombinant Lactobacillus acidophilus Expressing espA-Tir-M Confers Protection against Enterohemorrhagic Escherichia coli O157:H7 Challenge in Mice
IF: 4.076 -
Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model
IF: 2.662 -
Intranasal immunization with novel EspA-Tir-M fusion protein induces protective immunity against enterohemorrhagic Escherichia coli O157:H7 challenge in mice
IF: 2.009 -
Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis
IF: 5.008 -
Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice
IF: 2.319 -
Immunopathological assessments of human Blastocystis spp. in experimentally infected immunocompetent and immunosuppresed mice
IF: 2.027Journal： PARASITOLOGY RESEARCH (2016)PMID：26860840Reactivity：MouseSample Type：Serum,Tissue homogenate
实验操作视频
