Mouse IFN-γ(Interferon Gamma) ELISA Kit
中文名称：小鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFN-γ抗体与结合在包被抗体上的小鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice
IF: 6.313 -
Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
IF: 5.719 -
Chrysin inhibits hepatocellular carcinoma progression through suppressing programmed death ligand 1 expression
IF: 5.340 -
Reducing the Allergenicity of α-Lactalbumin after Lipid Peroxidation
IF: 5.279Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)PMID：33974424Reactivity：MouseSample Type：Cell culture supernatant -
Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway
IF: 4.932 -
The immunomodulatory effects of ginsenoside derivative Rh2-O on splenic lymphocytes in H22 tumor-bearing mice is partially mediated by TLR4
IF: 4.932 -
Attenuation of virulence in multiple serotypes (M1, M3, and M28) of Group A Streptococcus after the loss of secreted esterase
IF: 4.399Journal： JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION (2021)PMID：34674958Reactivity：MouseSample Type：skin lesion -
4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis
IF: 4.104 -
Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1-dependent inhibition of the NLRP3 inflammasome in an EAP mouse model
IF: 4.104 -
Molecular and functional characterization of HtrA protein in Actinobacillus pleuropneumoniae
IF: 3.293
实验操作视频
