Mouse IFN-γ(Interferon Gamma) ELISA Kit
中文名称：小鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-γ抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素，抗小鼠IFN-γ抗体与结合在包被抗体上的小鼠IFN-γ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IFN-γ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IFN-γ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Application of mouse model for evaluation of recombinant LpxC and GmhA as novel antigenic vaccine candidates of Glaesserella parasuis serotype 13
IF: 1.267Journal： JOURNAL OF VETERINARY MEDICAL SCIENCE (2021)PMID：34393140Reactivity：MouseSample Type：serum -
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
IF: 16.304Journal： Science Translational Medicine (2020)PMID：33239389Reactivity：MouseSample Type：Cell culture supernatant -
Engineering blood exosomes for tumor-targeting efficient gene/chemo combination therapy
IF: 8.579 -
Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis
IF: 5.778 -
Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells
IF: 5.162Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)PMID：32553971Reactivity：MouseSample Type：Cell sample -
CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis
IF: 4.966Journal： FASEB JOURNAL (2020)PMID：32862457Reactivity：MouseSample Type：Cell culture supernatant,Tissue homogenate,serum -
Cytotoxic T Cell Responses Induced by CS1/CRT Fusion DNA Vaccine in a Human Plasmacytoma Model
IF: 4.848 -
Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy
IF: 4.845Journal： INTERNATIONAL JOURNAL OF PHARMACEUTICS (2020)PMID：33010395Reactivity：MouseSample Type：Serum,Tissue homogenate -
Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function
IF: 4.546 -
Defibrotide inhibits donor leucocyte-endothelial interactions and protects against acute graft-versus-host disease
IF: 4.486Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020)PMID：32519822Reactivity：MouseSample Type：Plasma
实验操作视频
