Mouse IgM(Immunoglobulin M) ELISA Kit
中文名称：小鼠免疫球蛋白M(IgM)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Mouse
- 检测范围： 3.13-200 ng/mL
- 灵敏度： 1.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IgM抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IgM会与包被抗体结合。后依次加入生物素化的抗小鼠IgM抗体和辣根过氧化物酶标记的亲和素，抗小鼠IgM抗体与结合在包被抗体上的小鼠IgM结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IgM浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IgM的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 ng/mL |
| 灵敏度 | 1.88 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IgM，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
EZH2 promotes B-cell autoimmunity in primary Sjogren's syndrome via METTL3-mediated m6A modification
IF: 7.900Journal： JOURNAL OF AUTOIMMUNITY (2024)Reactivity：MouseSample Type：serumIQGAP1 promotes early B cell development, is essential for the development of marginal zone (MZ) B cells, and is critical for both T-dependent and T-independent antibody responses
IF: 6.200Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2024)Reactivity：MouseSample Type：seraExopolysaccharides and Surface-Layer Proteins Expressed by Biofilm-State Lactiplantibacillus plantarum Y42 Play Crucial Role in Preventing Intestinal Barrier and Immunity Dysfunction of Balb/C Mice Infected by Listeria monocytogenes ATCC 19115
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：serumEvaluation of the immunomodulatory effects of a novel synbiotic made of combined use of probiotic-prebiotic-Chinese traditional herbs
IF: 4.800Journal： Journal of Agriculture and Food Research (2024)Reactivity：MouseSample Type：serumMaternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
IF: 6.073Journal： Frontiers in Cellular and Infection Microbiology (2022)Reactivity：MouseSample Type：serum,stomach tissueMelatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses
IF: 4.171Betulinic Acid Attenuates Oxidative Stress in the Thymus Induced by Acute Exposure to T-2 Toxin via Regulation of the MAPK/Nrf2 Signaling Pathway
IF: 3.531Betulinic Acid Ameliorates the T-2 Toxin-Triggered Intestinal Impairment in Mice by Inhibiting Inflammation and Mucosal Barrier Dysfunction through the NF-κB Signaling Pathway
IF: 3.531Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota
IF: 2.581
