Mouse IL-10(Interleukin 10) ELISA Kit
中文名称：小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Epimedium polysaccharides ameliorate ulcerative colitis by inhibiting oxidative stress and regulating autophagy
IF: 3.3Journal： JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2024)Reactivity：MouseSample Type：colon tissue -
Sevoflurane blocks KLF5-mediated transcriptional activation of ITGB2 to inhibit macrophage infiltration in hepatic ischemia/reperfusion injury
IF: 3.2 -
Effects of topical isoxsuprine ointment on imiquimod-induced psoriasiform skin inflammation in mice
IF: 3.1Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：MouseSample Type：skin tissue -
In-vitro and in-vivo studies of two-drug cocktail therapy targeting chemobrain via the Nrf2/NF-κB signaling pathway
IF: 2.9Journal： JOURNAL OF MOLECULAR HISTOLOGY (2024)Reactivity：MouseSample Type：brain tissue -
Immuno-therapeutic and prophylactic potential of Trichinella spiralis antigens for inflammatory bowel diseases
IF: 2.9Journal： Food and Waterborne Parasitology (2024)Reactivity：MouseSample Type：serum -
Investigating the Phytochemical Composition, Antioxidant, and Anti-Inflammatory Potentials of Cassinopsis ilicifolia (Hochst.) Kuntze Extract against Some Oxidative Stress and Inflammation Molecular Markers
IF: 2.8 -
Coenzyme Q10 ameliorates chemotherapy-induced cognitive impairment in mice: a preclinical study
IF: 2.6Journal： MOLECULAR BIOLOGY REPORTS (2024)Reactivity：MouseSample Type：brain tissue -
Chlorophyll Derivatives Exert Greater Potency Over Progesterone in the Prevention of Infection-Induced Preterm Birth in Murine Models
IF: 2.5Journal： AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2024)Reactivity：MouseSample Type：uteru tissue,placenta tissue -
Ceftriaxone averts neuroinflammation and relieves depressive-like behaviors via GLT-1/TrkB signaling
IF: 2.5Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)Reactivity：MouseSample Type：hippocampal tissue -
Liang-Ge-San Decoction Ameliorates Acute Respiratory Distress Syndrome via Suppressing p38MAPK-NF-κ B Signaling Pathway
IF: 2.2Journal： Chinese Journal of Integrative Medicine (2024)Reactivity：Mouse,HumanSample Type：bronchoalveolar lavage fluid (BALF),lung tissue
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