Mouse IL-10(Interleukin 10) ELISA Kit
中文名称：小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Exosomes derived from synovial fibroblasts under hypoxia aggravate rheumatoid arthritis by regulating Treg/Th17 balance
IF: 3.139Journal： EXPERIMENTAL BIOLOGY AND MEDICINE (2020)PMID：32615822Reactivity：MouseSample Type：Cell culture medium -
Design and synthesis of 1,3-benzothiazinone derivatives as potential anti-inflammatory agents
IF: 3.073Journal： BIOORGANIC & MEDICINAL CHEMISTRY (2020)PMID：32354672Reactivity：MouseSample Type：Cell culture supernatant -
Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF?κB activation
IF: 2.914 -
Exposure time determines the protective effect of Trichinella spiralis on experimental colitis
IF: 2.914 -
Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom
IF: 2.762 -
IL-4 functionalized titanium dioxide nanotubes modulate the inflammatory response of macrophages
IF: 2.690 -
Activation of Paraventricular Melatonin Receptor 2 Mediates Melatonin-Conferred Cardioprotection Against Myocardial Ischemia/Reperfusion Injury
IF: 2.598Journal： JOURNAL OF CARDIOVASCULAR PHARMACOLOGY (2020)PMID：32433359Reactivity：MouseSample Type：PVN tissue -
The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis
IF: 2.379 -
NB-UVB irradiation attenuates inflammatory response in psoriasis
IF: 2.327 -
FSC231 can alleviate paclitaxel-induced neuralgia by inhibiting PICK1 and affecting related factors
IF: 2.274
实验操作视频
