Mouse IL-10(Interleukin 10) ELISA Kit
中文名称：小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Frequent exposure to varied home cage sizes alters pain sensitivity and some key inflammation-related biomarkers
IF: 2.214Journal： JOURNAL OF NEUROSCIENCE METHODS (2020)PMID：32768413Reactivity：MouseSample Type：Tissue homogenate -
MicroRNA-345-5p regulates depression by targeting suppressor of cytokine signaling 1
IF: 2.091Journal： Brain and Behavior (2020)PMID：32730696Reactivity：MouseSample Type：Cell culture supernatant -
Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth.
IF: 1.813Journal： Evidence-based Complementary and Alternative Medicine (2020)PMID：32655661Reactivity：Mouse -
Colonisation of mice and pigs by a chimeric porcine circovirus 1–2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines
IF: 1.786Journal： JOURNAL OF VIROLOGICAL METHODS (2020)PMID：32502500Reactivity：MouseSample Type：Cell culture supernatant -
Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate?induced colitis
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32934675Reactivity：MouseSample Type：Serum -
Oral administration of 3,3′-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis
IF: 5.657Journal： FREE RADICAL BIOLOGY AND MEDICINE (2019)PMID：31521664Reactivity：MouseSample Type：Bronchoalveolar Lavage Fluid,Tissue homogenate -
Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis
IF: 4.868Journal： Oxidative Medicine and Cellular Longevity (2019)PMID：31531179Reactivity：MouseSample Type：Tissue homogenate -
Severe Fever With Thrombocytopenia Syndrome Virus-Induced Macrophage Differentiation Is Regulated by miR-146
IF: 4.716 -
Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival
IF: 4.183Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)PMID：31340581Reactivity：MouseSample Type：Serum,Cell culture supernatant -
HMG-CoA Reductase Inhibitors Attenuate Neuronal Damage by Suppressing Oxygen Glucose Deprivation-Induced Activated Microglial Cells
IF: 3.591Journal： NEURAL PLASTICITY (2019)PMID：30911291Reactivity：MouseSample Type：Cell culture supernatant
实验操作视频
