Mouse IL-10(Interleukin 10) ELISA Kit
中文名称：小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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HMGB1/TREM2 positive feedback loop drives the development of radioresistance and immune escape of glioblastoma by regulating TLR4/Akt signaling
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：serum -
Encapsulation of telmisartan inside insulinoma-cell-derived extracellular vesicles outperformed biomimetic nanovesicles in modulating the pancreatic inflammatory microenvironment
IF: 6.100Journal： Journal of Materials Chemistry B (2024)Reactivity：MouseSample Type：serum -
A biomimetic solution, albumin–doxorubicin molecular complex, targeting tumor and tumor-draining lymph nodes
IF: 6.100Journal： Journal of Materials Chemistry B (2024)Reactivity：MouseSample Type：4T1 cell-Xenograft -
TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice
IF: 6.000Journal： MOLECULAR MEDICINE (2024)Reactivity：MouseSample Type：spleen tissue -
Exopolysaccharides and Surface-Layer Proteins Expressed by Biofilm-State Lactiplantibacillus plantarum Y42 Play Crucial Role in Preventing Intestinal Barrier and Immunity Dysfunction of Balb/C Mice Infected by Listeria monocytogenes ATCC 19115
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：serum -
Dimethyl fumarate alleviates allergic asthma by strengthening the Nrf2 signaling pathway in regulatory T cells
IF: 5.700Journal： Frontiers in Immunology (2024)Reactivity：MouseSample Type：lung tissue -
Palmatine Attenuated Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting M1 Phenotype Macrophage Polarization via NAMPT/TLR2/CCR1 Signaling
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：Bronchoalveolar lavage fluids (BALFs),serum -
Delivery of exogenous miR-19b by Wharton’s Jelly Mesenchymal Stem Cells attenuates transplanted kidney ischemia/reperfusion injury by regulating cellular metabolism
IF: 5.700Journal： Drug Delivery and Translational Research (2024)Reactivity：MouseSample Type：serum -
Rice Protein Peptides Alleviate Alcoholic Liver Disease via the PPARγ Signaling Pathway: Through Liver Metabolomics and Gut Microbiota Analysis
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：liver tissue -
Sustained delivery of NT-3 and curcumin augments microenvironment modulation effects of decellularized spinal cord matrix hydrogel for spinal cord injury repair
IF: 5.600
实验操作视频
