Mouse IL-10(Interleukin 10) ELISA Kit
中文名称：小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
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IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：Serum,Lung tissue -
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Effect of tangeretin on cisplatin-induced oxido-inflammatory brain damage in rats
IF: 4.300Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2024)Reactivity：MouseSample Type：brain tissue -
Kaempferol Alleviates Hepatic Injury in Nonalcoholic Steatohepatitis (NASH) by Suppressing Neutrophil-Mediated NLRP3-ASC/TMS1-Caspase 3 Signaling
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ML218 modulates calcium binding protein, oxidative stress, and inflammation during ischemia-reperfusion brain injury in mice
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：MouseSample Type：Brain tissue -
Co-exposure to multiple heavy metals and metalloid induces dose dependent modulation in antioxidative, inflammatory, DNA damage and apoptic pathways progressing to renal dysfunction in mice
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Modulating JAK2/STAT3 signaling by quercetin in Qiling Baitouweng Tang: a potential therapeutic approach for diffuse large B-cell lymphoma
IF: 3.900Journal： MOLECULAR DIVERSITY (2024)Reactivity：MouseSample Type：serum -
Integrative metabolomics and gut microbiota analyses reveal the protective effects of DHA-enriched phosphatidylserine on bisphenol A-induced intestinal damage
IF: 3.800Journal： Journal of Functional Foods (2024)Reactivity：MouseSample Type：jejunum supernatant -
Green Synthesis of Gold Nanoparticles from Polygahatous Polysaccharides and Their Anticancer Effect on Hepatic Carcinoma through Immunoregulation
IF: 3.700 -
Effects and Mechanisms of the Xianhecao-Huanglian Drug Pair on Autophagy-Mediated Intervention in Acute Inflammatory Bowel Disease via the JAK2/STAT3 Pathway
IF: 3.700Journal： BIOLOGICAL PROCEDURES ONLINE (2024)Reactivity：MouseSample Type：colon tissue,serum
实验操作视频
