Mouse IL-12(Interleukin 12) ELISA Kit
中文名称：小鼠白介素12(IL-12)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 pg/mL
- 灵敏度： 0.17 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-12抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-12会与包被抗体结合。后依次加入生物素化的抗小鼠IL-12抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-12抗体与结合在包被抗体上的小鼠IL-12结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-12浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-12的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 pg/mL |
| 灵敏度 | 0.17 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-12，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function
IF: 4.546 -
Defibrotide inhibits donor leucocyte-endothelial interactions and protects against acute graft-versus-host disease
IF: 4.486Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020)PMID：32519822Reactivity：MouseSample Type：Plasma -
Toxoplasma gondii ROP16I Deletion: The Exacerbated Impact on Adverse Pregnant Outcomes in Mice
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A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC
IF: 4.187Journal： Microbial Cell Factories (2020)PMID：32075660Reactivity：MouseSample Type：Cell culture supernatant -
Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis
IF: 4.092 -
Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway
IF: 3.998 -
Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma
IF: 3.924 -
Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection
IF: 2.914Journal： MICROBIAL PATHOGENESIS (2020)PMID：32781100Reactivity：MouseSample Type：Serum,Cell culture supernatant
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