Mouse IL-13(Interleukin 13) ELISA Kit
中文名称：小鼠白介素13(IL-13)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-13抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-13会与包被抗体结合。后依次加入生物素化的抗小鼠IL-13抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-13抗体与结合在包被抗体上的小鼠IL-13结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-13浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-13的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-13，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring
IF: 5.108 -
Suhuang Antitussive Capsules-Ameliorative Effects on LPS-Induced Sputum Obstruction in Mice Through Promoting HGF Secretion
IF: 3.845Journal： Frontiers in Pharmacology (2019)PMID：31920638Reactivity：MouseSample Type：Serum,Tissue homogenate -
C/EBP Homologous Protein (CHOP) Activates Macrophages and Promotes Liver Fibrosis in Schistosoma japonicum-Infected Mice
IF: 3.404 -
Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota
IF: 2.581Journal： MICROBIAL PATHOGENESIS (2019)PMID：31153984Reactivity：MouseSample Type：small intestinal tissues -
Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice
IF: 2.482 -
Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress
IF: 1.751Journal： RESEARCH IN VETERINARY SCIENCE (2019)PMID：31412308Reactivity：MouseSample Type：Serum,Tissue homogenate -
Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IκB/P38 MAPK signaling pathway
IF: 2.559Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018)PMID：30528734Reactivity：MouseSample Type：Tissue homogenate,Cell culture supernatant -
Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria
IF: 1.515 -
Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice
IF: 4.259Journal： Scientific Reports (2017)PMID：28225024Reactivity：MouseSample Type：Bronchoalveolar Lavage Fluid,Serum -
Mechanism of Corilagin interference with IL-13/STAT6 signaling pathways in hepatic alternative activation macrophages in schistosomiasis-induced liver fibrosis in mouse model
IF: 2.730
实验操作视频
