Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称：小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-17A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-17A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-17A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The contribution of IL-17A-dependent low LCN2 levels to Helicobacter pylori infection: Insights from clinical and experimental studies
IF: 5.600Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)PMID：37722259Reactivity：MouseSample Type：gastric tissues -
CD4+TEM cells drive the progression from acute myocarditis to dilated cardiomyopathy in CVB3-induced BALB/c mice
IF: 5.600 -
CKBA suppresses mast cell activation via ERK signaling pathway in murine atopic dermatitis
IF: 5.400 -
Effects of iguratimod on inflammatory factors and apoptosis of submandibular gland epithelial cells in NOD mice
IF: 4.600 -
Exploring the mechanisms of action of Zengye decoction (ZYD) against Sjogren’s syndrome (SS) using network pharmacology and animal experiment
IF: 3.800 -
Suppression of mir-150–5p attenuates the anti-inflammatory effect of glucocorticoids in mice with ulcerative colitis
IF: 3.600 -
Preclinical Short-term and Long-term Safety of Human Bone Marrow Mesenchymal Stem Cells
IF: 3.300 -
Enforced mesenchymal stem cell tissue colonization counteracts immunopathology
IF: 14.404 -
HA/CD44 Regulates the T Helper 1 Cells Differentiation by Activating Annexin A1/Akt/mTOR Signaling to Drive the Pathogenesis of EAP
IF: 8.786 -
Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate
IF: 8.786Journal： Frontiers in Immunology (2022)PMID：35860242Reactivity：MouseSample Type：serum,prostate tissue
实验操作视频
