Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称：小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-17A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-17A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-17A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Successful Treatment of Vitiligo with Cold Atmospheric Plasma?Activated Hydrogel
IF: 8.551Journal： JOURNAL OF INVESTIGATIVE DERMATOLOGY (2021)PMID：34029575Reactivity：MouseSample Type：Serum,Tissue homogenate -
Interleukin-17 upregulation participates in the pathogenesis of heart failure in mice via NF-κB-dependent suppression of SERCA2a and Cav1.2 expression
IF: 6.150Journal： ACTA PHARMACOLOGICA SINICA (2021)PMID：33589793Reactivity：MouseSample Type：Plasma,Tissue homogenate(heart) -
Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice
IF: 5.924 -
Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
IF: 5.719 -
Appetite Suppression and Interleukin 17 Receptor Signaling Activation of Colonic Mycobiota Dysbiosis Induced by High Temperature and High Humidity Conditions
IF: 5.293Journal： Frontiers in Cellular and Infection Microbiology (2021)PMID：34568080Reactivity：MouseSample Type：serum -
Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway
IF: 4.932 -
4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis
IF: 4.104 -
Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1-dependent inhibition of the NLRP3 inflammasome in an EAP mouse model
IF: 4.104 -
MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2
IF: 2.611Journal： JOURNAL OF MOLECULAR HISTOLOGY (2021)PMID：34623552Reactivity：MouseSample Type：colonic tissue -
MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy
IF: 6.304
实验操作视频
