Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称：小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-17A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-17A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-17A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice.
IF: 1.273 -
Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model
IF: 4.259Journal： Frontiers in Microbiology (2019)PMID：31333606Reactivity：MouseSample Type：Tissue homogenate -
Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-na?ve rheumatoid arthritis patients
IF: 3.361 -
Gastrodin alleviates Tourette syndrome via Nrf-2/HO-1/HMGB1/NF-кB pathway
IF: 2.965Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019)PMID：31468582Reactivity：RatSample Type：Tissue homogenate -
Role of Interleukin-17 in Pathogenesis of Intestinal Fibrosis in Mice
IF: 2.937Journal： DIGESTIVE DISEASES AND SCIENCES (2019)PMID：31808003Reactivity：MouseSample Type：blood, colonic tissue -
1,25(OH)2D3 inhibited Th17 cells differentiation via regulating the NF-κB activity and expression of IL-17
IF: 4.936 -
RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice
IF: 2.884 -
AT1-receptor blockade: Protective effects of irbesartan in cardiomyocytes under hypoxic stress
IF: 2.766 -
Correlation between serum IL-17A level and SALT score in patients with alopecia areata before and after NB-UVB therapy
IF: 1.529 -
Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis
IF: 5.168
实验操作视频
