Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称：小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-17A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-17A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-17A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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IF: 5.719 -
Appetite Suppression and Interleukin 17 Receptor Signaling Activation of Colonic Mycobiota Dysbiosis Induced by High Temperature and High Humidity Conditions
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IF: 4.104 -
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MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy
IF: 6.304 -
Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis
IF: 5.778 -
Divergent Effect of Tacalcitol (PRI-2191) on Th17 Cells in 4T1 Tumor Bearing Young and Old Ovariectomized Mice
IF: 5.402 -
CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis
IF: 4.966Journal： FASEB JOURNAL (2020)PMID：32862457Reactivity：MouseSample Type：Cell culture supernatant,Tissue homogenate,serum
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