Mouse IL-18(Interleukin 18) ELISA Kit
中文名称：小鼠白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-18抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-18会与包被抗体结合。后依次加入生物素化的抗小鼠IL-18抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-18抗体与结合在包被抗体上的小鼠IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Potential Mechanisms of Aortic Medial Degeneration Promoted by Co-Exposure to Microplastics and Lead
IF: 12.200Journal： JOURNAL OF HAZARDOUS MATERIALS (2024)Reactivity：MouseSample Type：serum -
ADAM8 deficiency in macrophages promotes cardiac repair after myocardial infarction via ANXA2-mTOR-autophagy pathway
IF: 11.400 -
Neutrophil extracellular traps facilitate liver inflammation/fibrosis progression by entering macrophages and triggering AIM2 inflammasome-dependent pyroptosis
IF: 8.200Journal： Cell Communication and Signaling (2024)Reactivity：MouseSample Type：chronic hepatitis (CH) biopsy tissue,serum -
CircHipk3 serves a dual role in macrophage pyroptosis by promoting NLRP3 transcription and inhibition of autophagy to induce abdominal aortic aneurysm formation
IF: 7.900 -
Cecal necroptosis triggers lethal cardiac dysfunction in TNF-induced severe SIRS
IF: 7.500 -
Gentiopicroside improves NASH and liver fibrosis by suppressing TLR4 and NLRP3 signaling pathways
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：MouseSample Type：serum,liver tissue -
Rab11b promotes M1-like macrophage polarization by restraining autophagic degradation of NLRP3 in alcohol-associated liver disease
IF: 6.900 -
Rhizoma Alismatis Decoction improved mitochondrial dysfunction to alleviate SASP by enhancing autophagy flux and apoptosis in hyperlipidemia acute pancreatitis
IF: 6.700 -
Short-term respiratory cadmium exposure partially activates pulmonary NLRP3 inflammasome by inducing ferroptosis in mice
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：MouseSample Type：Lung tissue -
Atox1 regulates macrophage polarization in intestinal inflammation via ROS-NLRP3 inflammasome pathway
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：colon tissue
实验操作视频
