Mouse IL-18(Interleukin 18) ELISA Kit
中文名称：小鼠白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-18抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-18会与包被抗体结合。后依次加入生物素化的抗小鼠IL-18抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-18抗体与结合在包被抗体上的小鼠IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways
IF: 4.123Journal： Frontiers in Cellular and Infection Microbiology (2020)PMID：32432055Reactivity：MouseSample Type：Cell culture supernatant -
COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：32702600Reactivity：MouseSample Type：Tissue homogenate -
Adiponectin Inhibits NLRP3 Inflammasome Activation in Nonalcoholic Steatohepatitis via AMPK-JNK/ErK1/2-NFκB/ROS Signaling Pathways
IF: 3.900Journal： Frontiers in Medicine (2020)PMID：33251225Reactivity：MouseSample Type：Tissue homogenate(liver tissue) -
Ablation of GSDMD Improves Outcome of Ischemic Stroke Through Blocking Canonical and Non-canonical Inflammasomes Dependent Pyroptosis in Microglia
IF: 2.889 -
hnRNPK knockdown alleviates NLRP3 inflammasome priming by repressing FLIP expression in Raw264.7 macrophages
IF: 2.753 -
Sodium P-aminosalicylic Acid Inhibits Manganese-Induced Neuroinflammation in BV2 Microglial Cells via NLRP3-CASP1 Inflammasome Pathway
IF: 2.639Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2020)PMID：33156491Reactivity：MouseSample Type：Cell sample -
miR-1929-3p Overexpression Alleviates Murine Cytomegalovirus-Induced Hypertensive Myocardial Remodeling by Suppressing Ednra/NLRP3 Inflammasome Activation
IF: 2.276Journal： Biomed Research International (2020)PMID：33457411Reactivity：MouseSample Type：serum, heart -
Mechanistic Insight Into the Activation of the NLRP3 Inflammasome by Neisseria gonorrhoeae in Macrophages
IF: 4.716 -
N-Acetyl cysteine effectively alleviates Coxsackievirus B-Induced myocarditis through suppressing viral replication and inflammatory response
IF: 4.130 -
Icariin targets Nrf2 signaling to inhibit microglia-mediated neuroinflammation
IF: 3.361
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
