Mouse IL-18(Interleukin 18) ELISA Kit
中文名称：小鼠白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-18抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-18会与包被抗体结合。后依次加入生物素化的抗小鼠IL-18抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-18抗体与结合在包被抗体上的小鼠IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Rhizoma Alismatis Decoction improved mitochondrial dysfunction to alleviate SASP by enhancing autophagy flux and apoptosis in hyperlipidemia acute pancreatitis
IF: 6.7 -
Short-term respiratory cadmium exposure partially activates pulmonary NLRP3 inflammasome by inducing ferroptosis in mice
IF: 6.2Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：MouseSample Type：Lung tissue -
Atox1 regulates macrophage polarization in intestinal inflammation via ROS-NLRP3 inflammasome pathway
IF: 6.1Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：colon tissue -
Palmatine Attenuated Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting M1 Phenotype Macrophage Polarization via NAMPT/TLR2/CCR1 Signaling
IF: 5.7Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：Bronchoalveolar lavage fluids (BALFs),serum -
S1PR2 participates in intestinal injury in severe acute pancreatitis by regulating macrophage pyroptosis
IF: 5.7Journal： Frontiers in Immunology (2024)Reactivity：MouseSample Type：serum -
Vitamin D3 improves glucose metabolism and attenuates inflammation in prediabetic human and mice
IF: 4.8Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2024)Reactivity：MouseSample Type：serum -
AdipoRon exerts an antidepressant effect by inhibiting NLRP3 inflammasome activation in microglia via promoting mitophagy
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：hippocamp tissue -
Stachyose ameliorates myocardial ischemia–reperfusion injury by inhibiting cardiomyocyte ferroptosis and macrophage pyroptosis
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Inhibiting endoplasmic reticulum stress alleviates perioperative neurocognitive disorders by reducing neuroinflammation mediated by NLRP3 inflammasome activation
IF: 4.8Journal： CNS Neuroscience & Therapeutics (2024)Reactivity：MouseSample Type：hippocampal tissue -
An Amino Acids and Dipeptide Injection Inhibits the TNF-α/HMGB1 Inflammatory Signaling Pathway to Reduce Pyroptosis and M1 Microglial Polarization in POCD Mice: the Gut to the Brain
IF: 4.6Journal： MOLECULAR NEUROBIOLOGY (2024)Reactivity：MouseSample Type：serum
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