Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称:小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-1β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-1β的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠IL-1β,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
A Customized Biohybrid presenting Cascade Responses to Tumor Microenvironment
IF: 27.400 -
Hydrolysis of 2D Nanosheets Reverses Rheumatoid Arthritis Through Anti-Inflammation and Osteogenesis
IF: 27.400 -
GSNOR negatively regulates the NLRP3 inflammasome via S-nitrosation of MAPK14
IF: 21.800Journal: Cellular & Molecular Immunology (2024)Reactivity:MouseSample Type:colonic tissue,serum -
Rewiring Tryptophan Metabolism via Programmable Probiotic Integrated by Dual-Layered Microcapsule Protects against Inflammatory Bowel Disease in Mice
IF: 15.800 -
Milk-derived extracellular vesicles functionalized with anti-tumour necrosis factor-α nanobody and anti-microbial peptide alleviate ulcerative colitis in mice
IF: 15.500Journal: Journal of Extracellular Vesicles (2024)Reactivity:MouseSample Type:colon tissue -
SHISA3 Reprograms Tumor-Associated Macrophages Toward an Antitumoral Phenotype and Enhances Cancer Immunotherapy
IF: 14.300 -
Targeting ATAD3A Phosphorylation Mediated by TBK1 Ameliorates Senescence-Associated Pathologies
IF: 14.300 -
Ruthenium(II) Lipid-Mimics Drive Lipid Phase Separation to Arouse Autophagy-Ferroptosis Cascade for Photoimmunotherapy
IF: 14.300 -
Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury
IF: 14.300Journal: Advanced Science (2024)Reactivity:MouseSample Type:kidney tissue -
Lactylation of Histone H3k18 and Egr1 Promotes Endothelial Glycocalyx Degradation in Sepsis-Induced Acute Lung Injury
IF: 14.300Journal: Advanced Science (2024)Reactivity:MouseSample Type:bronchoalveolar lavage fluid (BALF)
Q1:Mouse IL-1β elisa试剂盒检测的是precusor还是mature的IL-1β?
白细胞介素(IL)-1细胞因子家族与免疫反应和炎症相关。它由以下11个不同的分子形式组成:细胞间IL-1α (IL-1F1)、细胞外IL-1β (IL-1F2)、IL-1受体拮抗剂(IL-1Ra, IL-1F3),以及IL-18 (IL-1F4)、IL-33 (IL-1F11)、IL-36α(IL-1F6)、IL-36β(IL-1F8)、IL-36γ(IL-1F9)、IL-36Ra(IL-1F5)、IL-37 (IL-1F7)和IL-38 (L-1F10)。其中最重要的促炎细胞因子是IL-1β。它主要是由单核细胞和巨噬细胞产生的一种非活性物质31 kDa前体,白细胞介素-1β原(proIL-1β),在半胱氨酸蛋白酶IL -1转化酶(ICE)的作用下可生成一个17.5kDa成熟且具有生物活性的蛋白IL-1β,E-EL-M0037试剂盒检测的是成熟的IL-1β。