Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Olfactory mucosa mesenchymal stem cell-derived exosomes protect against neuroinflammation after subarachnoid hemorrhage by activating mitophagy
IF: 2.7Journal： KAOHSIUNG JOURNAL OF MEDICAL SCIENCES (2025)Reactivity：MouseSample Type：cortex tissue -
Transcranial pulsed current stimulation alleviates neuronal pyroptosis and neurological dysfunction following traumatic brain injury via the orexin-A/NLRP3 pathway
IF: 2.5Journal： NEUROPEPTIDES (2025)Reactivity：MouseSample Type：perilesional cortex tissue -
Guaiane sesquiterpenoid—Glucozaluzanin C from Ainsliaea fragrans Champ. targeting binding to TLR4/p65 to regulate NF-κB/MAPK pathway alleviates sepsis-induced acute liver injury
IF: 2.5 -
NSUN3 Aggravates Sepsis-Associated Acute Kidney Injury by Stabilising TIFA mRNA Through m5C
IF: 2.4Journal： CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2025)Reactivity：MouseSample Type：serum -
The relationship between “microbiota-gut-brain” axis and depression: Chronic stress-induced inflammation
IF: 2.4Journal： PHYSIOLOGY & BEHAVIOR (2025)Reactivity：MouseSample Type：hippocampus tissue,serum -
Antidepressant-Like Effects of Hydroxychavicol-Enriched Piper betle L. Leaf Extract in Chronic Mild Stress–Induced Rodent Models and Possible Mechanism(s) of Action Involved
IF: 2.3Journal： CHEMISTRY & BIODIVERSITY (2025)Reactivity：MouseSample Type：Serum -
Echinacoside attenuates Klebsiella pneumoniae-induced pneumonia via inhibition of the TLR4/NF-κB signaling
IF: 2.2Journal： APMIS (2025)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Kurarinone Attenuates LPS-Induced Pneumonia by Inhibiting MAPK and NF-κB Signaling Pathways
IF: 2.2 -
HBCOC attenuates cerebral ischemia-reperfusion injury in mice by inhibiting the inflammatory response and autophagy via TREM-1/ERK/NF-κB
IF: 2.0Journal： Journal of Stroke & Cerebrovascular Diseases (2025)Reactivity：MouseSample Type：plasma -
Circ_0000215 Aggravates Cerebral Ischemic Vertigo by Targeting miR-361-3p to Promote Neuroinflammation and Apoptosis
IF: 2Journal： Journal of Stroke & Cerebrovascular Diseases (2025)Reactivity：MouseSample Type：brain tissue
实验操作视频
