Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Modulation of tenofovir by probenecid: Impact on drug, interleukin-1β, and dopamine concentration in the prefrontal cortex and cerebellum
IF: 2.900Journal： NEUROSCIENCE (2024)Reactivity：MouseSample Type：brain tissue -
Hesperetin protects against rotenone-induced motor disability and neurotoxicity via the regulation of SIRT1/NLRP3 signaling
IF: 2.800Journal： TOXICOLOGY MECHANISMS AND METHODS (2024)Reactivity：MouseSample Type：striatum tissue -
Investigating the Phytochemical Composition, Antioxidant, and Anti-Inflammatory Potentials of Cassinopsis ilicifolia (Hochst.) Kuntze Extract against Some Oxidative Stress and Inflammation Molecular Markers
IF: 2.800 -
KaiXinSan improves learning and memory impairment by regulating cholesterol homeostasis in mice overloaded with 27-OHC
IF: 2.700Journal： JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2024)Reactivity：MouseSample Type：brain tissue -
Coloprotective effects of chebulic myrobalan extract by regulation of AMPK-SIRT1 signaling: a pharmacological and histopathological evaluation
IF: 2.700Journal： TISSUE & CELL (2024)Reactivity：MouseSample Type：colon tissue -
Paroxetine alleviates ulcerative colitis in mice via restoring intestinal microbiota homeostasis and metabolism
IF: 2.700Journal： BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY (2024)Reactivity：MouseSample Type：colon tissue -
Coenzyme Q10 ameliorates chemotherapy-induced cognitive impairment in mice: a preclinical study
IF: 2.600Journal： MOLECULAR BIOLOGY REPORTS (2024)Reactivity：MouseSample Type：brain tissue -
Chlorophyll Derivatives Exert Greater Potency Over Progesterone in the Prevention of Infection-Induced Preterm Birth in Murine Models
IF: 2.500Journal： AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2024)Reactivity：MouseSample Type：uteru tissue,placenta tissue -
ALKBH5 deletion ameliorates inflammation by regulating IRF3 signaling in an m6A-dependent manner after myocardial infarction
IF: 2.500Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)Reactivity：MouseSample Type：Serum -
Ceftriaxone averts neuroinflammation and relieves depressive-like behaviors via GLT-1/TrkB signaling
IF: 2.500Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)Reactivity：MouseSample Type：hippocampal tissue
实验操作视频
