Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The polysaccharide from Aralia continentalis Kitagawa enhances immune responses via activating the MAPKs and NF-κB signaling pathways in RAW 264.7 macrophages
IF: 5.2Journal： Chemical and Biological Technologies in Agriculture (2024)Reactivity：Mouse -
Enhanced oxidative stress aggravates BLM-induced pulmonary fibrosis by promoting cellular senescence through enhancing NLRP3 activation
IF: 5.2Journal： LIFE SCIENCES (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF),serum -
Activation of the HMGB1-TLR4 pathway impacts the functionality of bone marrow mesenchymal stem cells and disrupts macrophage polarization in immune thrombocytopenia
IF: 5.1 -
Evaluation of Vincamine Loaded with Silver Nanoparticles as a New Potential Therapeutic Agent Against Ehrlich’s Solid Carcinoma in Mice
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Broccoli alleviates metabolic-associated fatty liver disease through regulating gut flora and lipid metabolism via the FXR/LXR signaling pathway
IF: 5.1Journal： Food & Function (2024)Reactivity：MouseSample Type：Serum,hepatic tissue -
The Role of LC3-Associated Phagocytosis Inhibits the Inflammatory Response in Aspergillus fumigatus Keratitis
IF: 5.0Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2024)Reactivity：MouseSample Type：corneas tissue -
Enlarged perivascular spaces in alcohol-related brain damage induced by dyslipidemia
IF: 4.9Journal： JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM (2024)Reactivity：MouseSample Type：brain tissue -
Anti-Neuroinflammatory Effect of Ombuin from Rhamnus?erythroxylon Pall. Leaves in LPS-Induced BV-2 Microglia by Targeting Src and Suppressing the PI3K-AKT/NF-κB Signaling Pathway
IF: 4.9Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：Mouse -
Computational Analysis and Experimental Data Exploring the Role of Hesperetin in Ameliorating ADHD and SIRT1/Nrf2/Keap1/OH-1 Signaling
IF: 4.9Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：MouseSample Type：brain tissue -
NMDAR (2C) deletion in astrocytes relieved LPS-induced neuroinflammation and depression
IF: 4.8
实验操作视频
